Quantification of azide groups on a material surface and a biomolecule using a clickable and cleavable fluorescent compound

Rika Sakai; Hiroki Iguchi; Tatsuo Maruyama

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Quantification of azide groups on a material surface and a biomolecule using a clickable and cleavable fluorescent compound

机译：使用可点击和可裂解的荧光化合物对材料表面和生物分子上的叠氮基进行定量

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Here, we propose a novel method for quantifying azide groups on a solid surface and a protein using a clickable and cleavable fluorescent compound. The clickable and cleavable fluorescent compound conjugates with the azide groups on the material surface and the fluorophore is then liberated into the solvent via a cleavage reaction, which can be simply quantified with a conventional fluorometer.

机译：在这里，我们提出了一种使用可点击和可裂解的荧光化合物定量测定固体表面和蛋白质上叠氮化物基团的新方法。可点击和可裂解的荧光化合物与材料表面上的叠氮基团缀合，然后荧光团通过裂解反应释放到溶剂中，该裂解反应可以用常规荧光计简单地定量。

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《RSC Advances》 |2019年第8期|共5页
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Rika Sakai; Hiroki Iguchi; Tatsuo Maruyama;
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中图分类化学;
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1. Quantification of azide groups on a material surface and a biomolecule using a clickable and cleavable fluorescent compound [J] . Sakai Rika, Iguchi Hiroki, Maruyama Tatsuo RSC Advances . 2019,第8期

机译：使用可点击和可切割的荧光化合物定量材料表面和生物分子上的叠氮基团
2. Quantification of Amino Groups on Solid Surfaces Using Cleavable Fluorescent Compounds [J] . Shiota Saori, Yamamoto Shunsuke, Shimomura Ayane, Langmuir: The ACS Journal of Surfaces and Colloids . 2015,第32期

机译：使用可裂解的荧光化合物对固体表面上的氨基进行定量
3. Covalent Surface Functionalization of Calcium Phosphate Nanoparticles with Fluorescent Dyes by Copper-Catalysed and by Strain-Promoted Azide-Alkyne Click Chemistry [J] . Rojas-Sanchez Leonardo, Sokolova Viktoriya, Riebe Steffen, ChemNanoMat . 2019,第4期

机译：铜催化剂荧光染料的磷酸钙纳米粒子的共价表面官能化和应变促进的α-炔烃键入化学
4. Clickable silk: novel silk materials containing azide reactive groups [C] . Hidetoshi Teramoto, Mizuki Sasaki, Yasushi Tamada, World biomaterials congress . 2016

机译：可点击的丝绸：含有叠氮化物反应性基团的新型丝绸材料
5. Photopolymerization of Cu(I)-Catalyzed Azide-Alkyne Cycloaddition (CuAAC) 'Click'-Based Networks for New-Age Materials [D] . Shete, Abhishek Umakant. 2018

机译：Cu（I）催化的叠氮化物-炔烃环加成（CuAAC）基于“点击”的新型材料网络的光聚合
6. Cleavable Biotin Probes for Labeling of Biomolecules via the Azide – Alkyne Cycloaddition [O] . Janek Szychowski, Alborz Mahdavi, Jennifer J. L. Hodas, -1

机译：裂解生物素探针通过叠氮生物分子的标记 - 炔环
7. Quantification of azide groups on a material surface and a biomolecule using a clickable and cleavable fluorescent compound [O] . Rika Sakai, Hiroki Iguchi, Tatsuo Maruyama 2019

机译：使用可点击和可切割的荧光化合物定量材料表面和生物分子上的叠氮基团

Quantification of azide groups on a material surface and a biomolecule using a clickable and cleavable fluorescent compound

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