• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>RSC Advances >Normal vibrations of ternary DOPC/DPPC/cholesterol lipid bilayers by low-frequency Raman spectroscopy
【24h】

Normal vibrations of ternary DOPC/DPPC/cholesterol lipid bilayers by low-frequency Raman spectroscopy

机译：低频拉曼光谱法研究三元DOPC / DPPC /胆固醇脂质双层的正常振动

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A lipid bilayer containing a ternary mixture of low- and high-melting lipids and cholesterol (Chol) can give rise to domain formation, referred to as lipid rafts. Low-frequency Raman spectroscopy at reduced temperatures allows detection of normal membrane mechanical vibrations. In this work, Raman spectra were obtained in the spectral range between 5 and 90 cm ~(?1) for bilayers prepared from dioleoyl- glycero -phosphocholine (DOPC), dipalmitoyl- glycero -phosphocholine (DPPC) and Chol. A narrow peak detected between 13 and 16 cm ~(?1) was attributed to the vibrational eigenmode of a lipid monolayer (a leaflet). For the equimolar DOPC/DPPC ratio, the Chol concentration dependence for the peak position, width and amplitude may be divided into three distinct ranges: below 9 mol%, the intermediate range between 9 mol% and 38 mol%, and above 38 mol%. In the intermediate range the peak position attains its minimum, and the peak width drops approximately by a factor of two as compared with the Chol-free bilayers. Meanwhile, this range is known for raft formation in a fluid state. The obtained results may be interpreted as evidence that bilayer structures in the raft-containing fluid state may be frozen at low temperatures. The drop of peak width indicates that at the spatial scale of the experiment (～2.5 nm) the intermolecular bilayer structure with raft formation becomes more homogeneous and more cohesive.
机译：包含低熔点和高熔点脂质与胆固醇（Chol）的三元混合物的脂质双层可以引起域形成，称为脂质筏。降低温度的低频拉曼光谱可以检测正常的膜片机械振动。在这项工作中，对于由二油酰基-甘油-磷酸胆碱（DOPC），二棕榈酰基-甘油-磷酸胆碱（DPPC）和Chol制备的双层，获得了在5至90cm 2（λ1）的光谱范围内的拉曼光谱。在13至16 cm〜（？1）之间检测到一个窄峰，这是由于脂质单层（小叶）的振动本征模引起的。对于等摩尔的DOPC / DPPC比，对于峰位置，宽度和幅度的Chol浓度依赖性可以分为三个不同的范围：低于9 mol％，介于9 mol％和38 mol％之间的中间范围以及高于38 mol％ 。在中间范围内，峰位置达到其最小值，并且与不含Chol的双层相比，峰宽下降了大约两倍。同时，该范围对于流体状态的筏形成是已知的。所获得的结果可以解释为证​​据，表明在低温下可以冻结含有筏的流体状态的双层结构。峰宽的下降表明，在实验的空间尺度上（约2.5 nm），具有筏形成的分子间双层结构变得更加均匀和凝聚。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号