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首页> 外文期刊>RSC Advances >Structural characterization of Momordica charantia?L. (Cucurbitaceae) oligopeptides and the detection of their capability in non-small cell lung cancer A549 cells: induction of apoptosis
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Structural characterization of Momordica charantia?L. (Cucurbitaceae) oligopeptides and the detection of their capability in non-small cell lung cancer A549 cells: induction of apoptosis

机译：苦瓜的结构表征（葫芦科）寡肽及其在非小细胞肺癌A549细胞中的能力检测：诱导凋亡

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摘要

Oligopeptides are rarely reported from Chinese herbal medicine because they are often present in very low concentrations in a complex matrix. Twenty-eight oligopeptides were recently identified by high-performance liquid chromatography and quadrupole-time-of-flight-mass spectrometry (HPLC-Q-TOF-MS) from Momordica charantia L. (Cucurbitaceae), and a septapeptide, FHGKGHE (Phe-His-Gly-Lys-Gly-His-Glu), named MCLO-12, showed the best anticancer activity against the non-small cell lung cancer A549 cell line in vitro , with an IC _(50) value of 21.4 ± 2.21 mM. The anti-proliferative activity assay results showed that MCLO-12 induced apoptosis of A549 cells in a concentration-dependent manner. Treatment of the cells with MCLO-12 (10.7–42.8 mM mL ~(?1) ) caused strong intracellular reactive oxygen species (ROS) up-regulating activities and activated caspase expression. MCLO-12 also suppressed the Trx system and subsequently activated a number of Trx-dependent pathways, including the ASK1, MAPK-p38 and JNK pathways. Thus, our research provides a good reference point for anti-NSCLC research into oligopeptides.
机译：很少从中草药报道寡肽，因为它们通常以非常低的浓度存在于复杂的基质中。最近通过高效液相色谱和苦瓜（Cucurbitaceae）的四极杆飞行时间质谱（HPLC-Q-TOF-MS）以及七肽FHGKGHE（Phe-名为MCLO-12的His-Gly-Lys-Gly-His-Glu）在体外对非小细胞肺癌A549细胞系表现出最佳的抗癌活性，IC _（50）值为21.4±2.21 mM 。抗增殖活性测定结果表明，MCLO-12以浓度依赖性方式诱导A549细胞凋亡。用MCLO-12（10.7–42.8 mM mL〜（？1））处理细胞会导致强烈的细胞内活性氧（ROS）上调活性并激活caspase表达。 MCLO-12还抑制了Trx系统，并随后激活了许多Trx依赖性途径，包括ASK1，MAPK-p38和JNK途径。因此，我们的研究为寡肽的抗NSCLC研究提供了很好的参考。

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