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首页> 外文期刊>RSC Advances >Polyethylenimine-alginate nanocomposites based bone morphogenetic protein 2 gene-activated matrix for alveolar bone regeneration
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Polyethylenimine-alginate nanocomposites based bone morphogenetic protein 2 gene-activated matrix for alveolar bone regeneration

机译：基于聚乙烯亚胺-海藻酸钠纳米复合材料的骨形态发生蛋白2基因激活基质用于牙槽骨再生

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The repair and treatment of lost or damaged alveolar bone is of great significance in dentistry. Gene-activated matrix (GAM) technology provides a new way for bone regeneration. It is a local gene delivery system, which can not only recruit cells, but also influence their fate. For this purpose, we fabricated a bone morphogenetic protein 2 ( BMP-2 ) gene-loaded absorbable gelatin sponge (AGS) and studied its effect on promoting alveolar bone formation and preventing resorption following tooth extraction in rats. In order to obtain better transfection efficiency, polyethylenimine-alginate (PEI-al) nanocomposites were synthesized and used as gene vectors to deliver BMP-2 cDNA plasmids (PEI-al/pBMP-2). The transfection efficiency, BMP-2 protein expression and osteogenic differentiation of the cells were investigated in vitro . In vivo , we established an alveolar bone regeneration model by extracting the rats' left mandibular incisors. The rats were randomly assigned into 3 groups: control group, unfilled sockets; AGS group, sockets filled with PEI-al solution-loaded gelatin sponges; AGS/BMP group, sockets filled with PEI-al/pBMP-2 solution-loaded gelatin sponge. Radiological and histological assays were performed at 4 and 8 weeks later. In vitro transfection assays indicated that PEI-al/pBMP-2 complexes could effectively transfect MC3T3-E1 cells, promoting the secretion of BMP-2 protein for at least 14 days, as well as increasing the expression of osteogenesis-related gene, ALP activity and calcium deposition. In vivo , western blot analysis showed BMP-2 protein was expressed in bone tissues of AGS/BMP group. The relative height of the residual alveolar ridge and bone mineral density (BMD) of the AGS/BMP group were significantly greater than those in the AGS and control groups at 4 and 8 weeks, respectively. Histological examination showed that, at 4 weeks, osteoblasts had grown in a cubic shape around the new bone in the AGS/BMP group, suggesting new bone formation. In conclusion, the combination of PEI-al/pBMP-2 complexes and gelatin sponge could promote alveolar bone regeneration, which may provide an easy and valuable method for alveolar ridge preservation and augmentation.
机译：牙槽骨丢失或损坏的修复和治疗在牙科领域具有重要意义。基因激活基质（GAM）技术为骨骼再生提供了一种新方法。这是一个局部基因传递系统，它不仅可以募集细胞，而且可以影响它们的命运。为此，我们制备了骨形态发生蛋白2（BMP-2）基因负载的可吸收明胶海绵（AGS），并研究了其在大鼠拔牙后促进牙槽骨形成和防止吸收的作用。为了获得更好的转染效率，合成了聚乙烯亚胺-海藻酸盐（PEI-al）纳米复合材料，并将其用作基因载体以递送BMP-2 cDNA质粒（PEI-al / pBMP-2）。体外研究了细胞的转染效率，BMP-2蛋白表达和成骨分化。在体内，我们通过提取大鼠左下颌切牙建立了牙槽骨再生模型。将大鼠随机分为3组：对照组，未装满牙套的组;和未装药的组。 AGS组，插座装有PEI-al溶液明胶海绵; AGS / BMP组，插座装有PEI-al / pBMP-2溶液明胶海绵。在第4和第8周后进行放射学和组织学测定。体外转染实验表明，PEI-al / pBMP-2复合物可以有效转染MC3T3-E1细胞，促进BMP-2蛋白的分泌至少14天，并增加成骨相关基因，ALP活性的表达。和钙沉积。体内Western blot分析显示，AGS / BMP组的骨组织中表达了BMP-2蛋白。 AGS / BMP组的残余牙槽相对高度和骨矿物质密度（BMD）分别在4周和8周时显着高于AGS和对照组。组织学检查显示，在第4周，成骨细胞在AGS / BMP组的新骨周围呈立方体状生长，表明有新的骨形成。总之，PEI-al / pBMP-2复合物与明胶海绵的组合可以促进牙槽骨的再生，这可能为牙槽的保存和增强提供一种简单而有价值的方法。

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