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首页> 外文期刊>RSC Advances >Polyethylenimine-alginate nanocomposites based bone morphogenetic protein 2 gene-activated matrix for alveolar bone regeneration
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Polyethylenimine-alginate nanocomposites based bone morphogenetic protein 2 gene-activated matrix for alveolar bone regeneration

机译：基于聚乙烯 - 藻酸盐纳米复合材料基于肺泡骨再生的骨形态发生蛋白2基因活化基质

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The repair and treatment of lost or damaged alveolar bone is of great significance in dentistry. Gene-activated matrix (GAM) technology provides a new way for bone regeneration. It is a local gene delivery system, which can not only recruit cells, but also influence their fate. For this purpose, we fabricated a bone morphogenetic protein 2 (BMP-2) gene-loaded absorbable gelatin sponge (AGS) and studied its effect on promoting alveolar bone formation and preventing resorption following tooth extraction in rats. In order to obtain better transfection efficiency, polyethylenimine-alginate (PEI-al) nanocomposites were synthesized and used as gene vectors to deliver BMP-2 cDNA plasmids (PEI-al/pBMP-2). The transfection efficiency, BMP-2 protein expression and osteogenic differentiation of the cells were investigated in vitro. In vivo, we established an alveolar bone regeneration model by extracting the rats' left mandibular incisors. The rats were randomly assigned into 3 groups: control group, unfilled sockets; AGS group, sockets filled with PEI-al solution-loaded gelatin sponges; AGS/BMP group, sockets filled with PEI-al/pBMP-2 solution-loaded gelatin sponge. Radiological and histological assays were performed at 4 and 8 weeks later. In vitro transfection assays indicated that PEI-al/pBMP-2 complexes could effectively transfect MC3T3-E1 cells, promoting the secretion of BMP-2 protein for at least 14 days, as well as increasing the expression of osteogenesis-related gene, ALP activity and calcium deposition. In vivo, western blot analysis showed BMP-2 protein was expressed in bone tissues of AGS/BMP group. The relative height of the residual alveolar ridge and bone mineral density (BMD) of the AGS/BMP group were significantly greater than those in the AGS and control groups at 4 and 8 weeks, respectively. Histological examination showed that, at 4 weeks, osteoblasts had grown in a cubic shape around the new bone in the AGS/BMP group, suggesting new bone formation. In conclusion, the combination of PEI-al/pBMP-2 complexes and gelatin sponge could promote alveolar bone regeneration, which may provide an easy and valuable method for alveolar ridge preservation and augmentation.
机译：损伤或受损的肺泡骨的修复和治疗在牙科方面具有重要意义。基因激活的矩阵（GAM）技术为骨再生提供了一种新的方式。它是一种局部基因递送系统，不仅可以招募细胞，而且影响其命运。为此目的，我们制造了骨形态发生蛋白2（BMP-2）最基载物吸收明胶海绵（AGS），并研究了其对大鼠齿提取后促进肺泡骨形成和防止吸收的影响。为了获得更好的转染效率，合成聚乙酰氨基氨基氨基酸盐（PEI-Al）纳米复合材料并用作基因载体，以递送BMP-2 cDNA质粒（PEI-Al / PBMP-2）。在体外研究转染效率，BMP-2蛋白表达和细胞的骨质发生分化。在体内，我们通过提取大鼠左下颌骨切牙来建立肺泡骨再生模型。将大鼠随机分配到3组：对照组，未填充插座; AGS组，插座充满了Pei-Al溶液加载的明胶海绵; AGS / BMP组，插座填充PEI-Al / PBMP-2溶液加载的明胶海绵。在4和8周后进行放射学和组织学测定。体外转染检测结果表明，PEI-Al / PBMP-2络合物可以有效地转染MC3T3-E1细胞，促进BMP-2蛋白的分泌至少14天，以及增加骨开发相关基因，ALP活性的表达和钙沉积。在体内，Western印迹分析显示BMP-2蛋白在AGS / BMP组的骨组织中表达。 AGS / BMP组的残留肺泡脊和骨密度（BMD）的相对高度分别分别在4和8周中显着大于AGS和对照组中的相对高度。组织学检查表明，在4周，成骨细胞在AGS / BMP组的新骨周围呈现出立方形状，表明新的骨形成。总之，Pei-Al / PBMP-2络合物和明胶海绵的组合可以促进肺泡骨再生，这可以为肺泡脊固化和增强提供一种简单且有价值的方法。

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    《RSC Advances》 |2019年第46期|共11页
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