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首页> 外文期刊>RSC Advances >Enantiopure polythiophene nanoparticles. Chirality dependence of cellular uptake, intracellular distribution and antimicrobial activity
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Enantiopure polythiophene nanoparticles. Chirality dependence of cellular uptake, intracellular distribution and antimicrobial activity

机译：对映纯聚噻吩纳米颗粒。细胞吸收，细胞内分布和抗菌活性的手性依赖性

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The use of intrinsic chiral molecules opens the door to bio-imaging specific tools and to the development of target-therapy. In this work the synthesis and characterization of polythiophenes with alkyl side chains containing one R or S chiral carbon is reported. Enantiopure chiral nanoparticles ( R or S NPs) were prepared from the polymers by a reprecipitation method. UV-vis, photoluminescence and circular dichroism spectroscopy of the NPs are described. In vitro analysis and metabolic assays show that both R and S NPs are efficiently taken-up by fibroblast cells without signs of toxicity. SDS-PAGE experiments show that formation of hard protein ‘corona’ enhances the chirality difference between nanoparticles. Co-localization experiments demonstrate that the cells are able to discriminate between the enantiomeric R and S nanoparticles. Finally, experiments carried out on Gram negative and Gram positive bacteria show that the enantiomeric NPs display different antibacterial activity.
机译：固有手性分子的使用为生物成像专用工具和靶疗法的发展打开了大门。在这项工作中，报道了具有一个含一个R或S手性碳的烷基侧链的聚噻吩的合成和表征。通过再沉淀法从聚合物中制备对映纯手性纳米颗粒（R或S NPs）。描述了NP的紫外可见，光致发光和圆二色性。体外分析和代谢测定表明，R和S NP都被成纤维细胞有效吸收，没有毒性迹象。 SDS-PAGE实验表明，硬蛋白“电晕”的形成增强了纳米颗粒之间的手性差异。共定位实验表明，细胞能够区分对映体R和S纳米颗粒。最后，对革兰氏阴性和革兰氏阳性细菌进行的实验表明，对映体NPs具有不同的抗菌活性。

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