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首页> 外文期刊>RSC Advances >Biocompatible chitosan–collagen–hydroxyapatite nanofibers coated with platelet-rich plasma for regenerative engineering of the rotator cuff of the shoulder
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Biocompatible chitosan–collagen–hydroxyapatite nanofibers coated with platelet-rich plasma for regenerative engineering of the rotator cuff of the shoulder

机译：生物相容性壳聚糖-胶原-羟基磷灰石纳米纤维涂有富含血小板的血浆，可用于肩周袖的再生工程

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摘要

Over the last few decades, extraordinary progress has been accomplished in the field of bone tissue engineering. Containing an incredible number of growth factors required for the process of osteogenesis, platelet-rich plasma (PRP) has gained much interest. However, because of the conflicting results obtained in various investigations, its adequacy remains a riddle. Accordingly, in this paper, we explore the in vitro application of biocompatible chitosan–collagen–hydroxyapatite (CS–COLL–HAP) nanofibers coated with platelet-rich plasma (PRP) (CS–COLL–HAP/PRP) scaffolds for the regenerative engineering of the rotator cuff (RCF) of the shoulder. FTIR spectroscopy, XRD, SEM-EDX and HRTEM were performed to evaluate the characteristics of nanofibers. After confirmation of the physicochemical properties of nanofibers, the osteogenic capability of the scaffold was assessed by measuring the relative calcium content, ALP activity, and gene expression. The results of viability and live/dead assay and cell adhesion test indicated the adequacy of the PRP when coupled with nanofibers in contrast to the other tested groups. In vivo staining affirmed increased collagen association in the PRP with nanofiber scaffolds at 30 days and 60 days. In conclusion, the addition of the PRP into CS–COLL–HAP nanofibers in this examination affected the osteogenic differentiation of osteoblast cells, and therefore, it may have an incredible perspective for bone tissue applications.
机译：在过去的几十年中，在骨组织工程领域已经取得了非凡的进步。富含血小板的血浆（PRP）包含成骨过程所需的大量生长因子，引起了人们的极大兴趣。但是，由于各种研究得出的结果相互矛盾，因此其充分性仍然是一个谜。因此，在本文中，我们探索了涂覆有富血小板血浆（PRP）（CS-COLL-HAP / PRP）支架的生物相容性壳聚糖-胶原-羟基磷灰石（CS-COLL-HAP）纳米纤维在体外的再生工程应用肩袖的长度（RCF）。进行了FTIR光谱，XRD，SEM-EDX和HRTEM评估纳米纤维的特性。确认纳米纤维的理化特性后，通过测量相对钙含量，ALP活性和基因表达来评估支架的成骨能力。活力，活/死分析和细胞粘附测试的结果表明，与其他测试组相比，当与纳米纤维偶联时，PRP是否足够。体内染色证实在30天和60天时，使用纳米纤维支架的PRP中的胶原缔合增加。总之，在这项检查中将PRP添加到CS–COLL–HAP纳米纤维中会影响成骨细胞的成骨分化，因此，对于骨组织的应用可能具有令人难以置信的前景。

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