• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>RSC Advances >Biocompatible chitosan-collagen-hydroxyapatite nanofibers coated with platelet-rich plasma for regenerative engineering of the rotator cuff of the shoulder
【24h】

Biocompatible chitosan-collagen-hydroxyapatite nanofibers coated with platelet-rich plasma for regenerative engineering of the rotator cuff of the shoulder

机译：生物相容性壳聚糖 - 胶原蛋白 - 羟基磷灰石纳米纤维含有富血小板的血浆，用于肩部旋转箍的再生工程

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Over the last few decades, extraordinary progress has been accomplished in the field of bone tissue engineering. Containing an incredible number of growth factors required for the process of osteogenesis, platelet-rich plasma (PRP) has gained much interest. However, because of the conflicting results obtained in various investigations, its adequacy remains a riddle. Accordingly, in this paper, we explore the in vitro application of biocompatible chitosan-collagen-hydroxyapatite (CS-COLL-HAP) nanofibers coated with platelet-rich plasma (PRP) (CS-COLL-HAP/PRP) scaffolds for the regenerative engineering of the rotator cuff (RCF) of the shoulder. FTIR spectroscopy, XRD, SEM-EDX and HRTEM were performed to evaluate the characteristics of nanofibers. After confirmation of the physicochemical properties of nanofibers, the osteogenic capability of the scaffold was assessed by measuring the relative calcium content, ALP activity, and gene expression. The results of viability and live/dead assay and cell adhesion test indicated the adequacy of the PRP when coupled with nanofibers in contrast to the other tested groups. In vivo staining affirmed increased collagen association in the PRP with nanofiber scaffolds at 30 days and 60 days. In conclusion, the addition of the PRP into CS-COLL-HAP nanofibers in this examination affected the osteogenic differentiation of osteoblast cells, and therefore, it may have an incredible perspective for bone tissue applications.
机译：在过去的几十年中，在骨组织工程领域已经实现了非凡的进展。含有骨质发生过程所需的令人难以置信的生长因子，富含血小板的血浆（PRP）已经获得了很多兴趣。但是，由于各种调查中获得的结果相互矛盾，其充足性仍然是谜语。因此，在本文中，我们探讨了涂有富含血小板血浆（PRP）（CS-Coll-Hap / PRP）支架的生物相容性壳聚糖 - 胶原醇 - 羟基磷灰石（CS-Coll-Hap）纳米纤维的体外应用，用于再生工程肩部的旋转袖口（RCF）。进行FTIR光谱，XRD，SEM-EDX和HRTEM进行评估纳米纤维的特性。在确认纳米纤维的物理化学性质之后，通过测量相对钙含量，ALP活性和基因表达来评估支架的成沸性。活力和活/死测定和细胞粘附试验结果表明，与其他测试组相比，与纳米纤维相结合时，PRP的充分性。体内染色在30天和60天的纳米纤维支架中肯定的胶原蛋白酶增加。总之，在该检查中添加PRP进入CS-Coll-Hap纳米纤维，影响成骨细胞的骨质形成细胞，因此，它可能对骨组织应用具有令人难以置信的视角。

著录项

  • 来源
    《RSC Advances》 |2019年第46期|共8页
  • 作者

  • 作者单位
  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 化学;
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号