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首页> 外文期刊>RSC Advances >Biocatalytically active microgels by precipitation polymerization of N-isopropyl acrylamide in the presence of an enzyme
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Biocatalytically active microgels by precipitation polymerization of N-isopropyl acrylamide in the presence of an enzyme

机译：在酶的存在下通过N-异丙基丙烯酰胺的沉淀聚合而具有生物催化活性的微凝胶

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摘要

We present a novel protocol for the synthesis of enzymatically active microgels. The protocol is based on the precipitation polymerization of N -isopropylacrylamide (NIPAm) in the presence of an enzyme and a protein binding comonomer. A basic investigation on the influence of different reaction parameters such as monomer concentration and reaction temperature on the microgel size and size distribution is performed and immobilization yields are determined. Microgels exhibiting hydrodynamic diameters between 100 nm and 1 μm and narrow size distribution could be synthesized while about 31–44% of the enzyme present in the initial reaction mixture can be immobilized. Successful immobilization including a verification of enzymatic activity of the microgels is achieved for glucose oxidase (GOx) and 2-deoxy- D -ribose-5-phosphate aldolase (DERA). The thermoresponsive properties of the microgels are assessed and discussed in the light of activity evolution with temperature. The positive correlation of enzymatic activity with temperature for the GOx containing microgel originates from a direct interaction of the enzyme with the PNIPAm based polymer matrix whose magnitude is highly influenced by temperature.
机译：我们提出了一种酶活性微凝胶合成的新协议。该方案基于N-异丙基丙烯酰胺（NIPAm）在酶和蛋白质结合共聚单体存在下的沉淀聚合反应。对不同反应参数（如单体浓度和反应温度）对微凝胶尺寸和尺寸分布的影响进行了基础研究，并确定了固定产率。可以合成出水动力直径在100 nm至1μm之间且尺寸分布窄的微凝胶，而固定在初始反应混合物中的酶中约31–44％可以被合成。对于葡萄糖氧化酶（GOx）和2-脱氧-D-核糖-5-磷酸醛缩酶（DERA），成功实现了固定化，包括验证了微凝胶的酶活性。根据活性随温度的变化评估和讨论了微凝胶的热响应特性。含GOx的微凝胶的酶活性与温度的正相关关系起因于酶与PNIPAm基聚合物基质的直接相互作用，其大小受温度影响很大。

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