Biocatalytically active microgels by precipitation polymerization of N-isopropyl acrylamide in the presence of an enzyme

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Biocatalytically active microgels by precipitation polymerization of N-isopropyl acrylamide in the presence of an enzyme

机译：在酶存在下通过丙烯酰胺的析出聚合进行生物催化的微凝胶

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We present a novel protocol for the synthesis of enzymatically active microgels. The protocol is based on the precipitation polymerization of N-isopropylacrylamide (NIPAm) in the presence of an enzyme and a protein binding comonomer. A basic investigation on the influence of different reaction parameters such as monomer concentration and reaction temperature on the microgel size and size distribution is performed and immobilization yields are determined. Microgels exhibiting hydrodynamic diameters between 100 nm and 1 mu m and narrow size distribution could be synthesized while about 31-44% of the enzyme present in the initial reaction mixture can be immobilized. Successful immobilization including a verification of enzymatic activity of the microgels is achieved for glucose oxidase (GOx) and 2-deoxy-d-ribose-5-phosphate aldolase (DERA). The thermoresponsive properties of the microgels are assessed and discussed in the light of activity evolution with temperature. The positive correlation of enzymatic activity with temperature for the GOx containing microgel originates from a direct interaction of the enzyme with the PNIPAm based polymer matrix whose magnitude is highly influenced by temperature.

机译：我们提出了一种用于合成酶活性微凝胶的新方案。该方案基于酶和蛋白质结合共聚单体存在N-异丙基丙烯酰胺（NIPAM）的沉淀聚合。确定了对微凝胶尺寸和尺寸分布的单体浓度和反应温度等单体浓度和反应温度的影响的基本研究并确定固定产率。可以合成在100nm和1μm和1μm和1μm和窄尺寸分布之间的流体动力学直径的微凝胶，而初始反应混合物中存在的约31-44％的酶可以固定。为葡萄糖氧化酶（GOX）和2-脱氧-D-核糖-5-磷酸醛糖酶（DERA）实现了包括微凝胶的酶活性验证的成功固定。根据温度的活性进化评估和讨论微凝胶的热敏性质。酶活性与含有微凝胶的温度的酶活性源自酶的直接相互作用与泊磷酸基的聚合物基质，其幅度受温度高度影响。

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《RSC Advances》 |2019年第49期|共10页
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1. Biocatalytically active microgels by precipitation polymerization of N-isopropyl acrylamide in the presence of an enzyme [J] . RSC Advances . 2019,第49期

机译：在酶存在下通过丙烯酰胺的析出聚合进行生物催化的微凝胶
2. Biocatalytically active microgels by precipitation polymerization of N-isopropyl acrylamide in the presence of an enzyme [J] . Stefan Reinicke, Thilo Fischer, Julia Bramski, RSC Advances . 2019,第49期

机译：在酶的存在下通过N-异丙基丙烯酰胺的沉淀聚合而具有生物催化活性的微凝胶
3. Biocatalytically active microgels by precipitation polymerization of N-isopropyl acrylamide in the presence of an enzyme [J] . Stefan Reinicke, Thilo Fischer, Julia Bramski, RSC Advances . 2019,第49期

机译：在酶的存在下通过N-异丙基丙烯酰胺的沉淀聚合而具有生物催化活性的微凝胶
4. Radical Polymerization of Optically Active (S)-N-(2-Hydroxy-1-phenylethyl) acrylamide in the Presence of Lewis Acids [C] . Yuanqi Zhu, Xiaodong Xu, Lifen Zhang, The 5th symposium on molecular chirality of Chinese Chemical Society amp; International Chiral Meeting . 2012

机译：存在路易斯酸的光学活性（S）-N-（2-羟基-1-苯基乙基）丙烯酰胺的自由基聚合
5. Characterization of N-isopropyl acrylamide based polymers for pH sensing and metal ion binding. [D] . Oxenford, Leah R. 2006

机译：用于pH传感和金属离子结合的N-异丙基丙烯酰胺基聚合物的表征。
6. Tuning the Size of Thermoresponsive Poly(N-Isopropyl Acrylamide) Grafted Silica Microgels [O] . Nils Nun, Stephan Hinrichs, Martin A. Schroer, 2017

机译：调整热响应性聚（N-异丙基丙烯酰胺）接枝硅胶微凝胶的大小
7. Biocatalytically active microgels by precipitation polymerization of N-isopropyl acrylamide in the presence of an enzyme [O] . Stefan Reinicke, Thilo Fischer, Julia Bramski, 2019

机译：在酶存在下通过丙烯酰胺的析出聚合进行生物催化的微凝胶

Biocatalytically active microgels by precipitation polymerization of N-isopropyl acrylamide in the presence of an enzyme

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