One step DNA amplification of mammalian cells in picoliter microwell arrays

Wenwen Liu; Zhao Li; Yuanjie Liu; Qingquan Wei; Yong Liu; Lufeng Ren; Chenyu Wang; Yude Yu

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One step DNA amplification of mammalian cells in picoliter microwell arrays

机译：皮升微孔阵列中哺乳动物细胞的一步DNA扩增

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We developed a strategy for direct DNA amplification of single cells on a PEG-modified silica chip with 30?600 picoliter-sized microwells. HPV-positive cells in heterogeneous populations were successfully detected with high accuracy sensitivity as high as single copy.

机译：我们开发了一种在具有30？600皮升大小的微孔的PEG修饰硅胶芯片上直接扩增单细胞的策略。已成功检测到异种群体中的HPV阳性细胞，其准确度高达单拷贝。

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《RSC Advances》 |2019年第5期|共5页
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Wenwen Liu; Zhao Li; Yuanjie Liu; Qingquan Wei; Yong Liu; Lufeng Ren; Chenyu Wang; Yude Yu;
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中图分类化学;
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1. One step DNA amplification of mammalian cells in picoliter microwell arrays [J] . Liu Wenwen, Li Zhao, Liu Yuanjie, RSC Advances . 2019,第5期

机译：Picoliter Microwell阵列中哺乳动物细胞的一步扩增
2. Single-Cell Chemical Lysis Method for Analyses of Intracellular Molecules Using an Array of Picoliter-Scale Microwells [J] . Yasuhiro Sasuga, Tomoyuki Iwasawa, Kayoko Terada, Analytical chemistry . 2008,第23期

机译：单细胞化学裂解法分析使用微升级微孔阵列的细胞内分子
3. Synergic TiO2 photocatalysis and guanine photoreduction for silver deposition amplification: an ultrasensitive and high-throughput visualized colorimetric analysis strategy for anthrax DNAs in blood using a wettable microwells array [J] . Liu Huan, Feng Luping, Cai Yuanyuan, Journal of Materials Chemistry, B. materials for biology and medicine . 2018,第45期

机译：银沉积扩增的协同TiO2光催化和鸟嘌呤映射：使用可湿性微孔阵列的炭疽多腹DNA的超敏和高通量可视化比色分析策略
4. Non-Contact Transfer of Nanoliter and Picoliter Volumes Containing DNA, Protein, and Cells [C] . Mitchell Mutz, Richard Ellson Chips to Hits . 2002

机译：包含DNA，蛋白质和细胞的纳升和皮升体积的非接触转移
5. Massively Parallel Polymerase Cloning and Genome Sequencing of Single Cells Using the Microwell Displacement Amplification System (MIDAS). [D] . Gole, Jeffrey. 2013

机译：使用微孔置换扩增系统（MIDAS）大规模并行聚合酶克隆和单细胞基因组测序。
6. Highly parallel introduction of nucleic acids into mammalian cells grown in microwell arrays [O] . Tilak Jain, Ryan McBride, Steven Head, -1

机译：高度并行引入核酸导入在微孔阵列中生长的哺乳动物细胞
7. Highly parallel introduction of nucleic acids into mammalian cells grown in microwell arrays [O] . Tilak Jain, Ryan McBride, Steven Head, 2009

机译：将核酸的高度平行引入Microwell阵列生长的哺乳动物细胞中

One step DNA amplification of mammalian cells in picoliter microwell arrays

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