Hydrogels that mimic native tissues chemically and structurally have been increasingly sought for a wide variety of tissue engineering applications. Gelatin can be naturally derived from different sources and functionalized to fabricate hydrogels that exhibit high cytocompatibility and favorable biodegradable properties. The amino groups on the gelatin backbone can be substituted by adding varying proportions of methacrylic anhydride (MAA) to create biomimetic hydrogels which can be used as tissue engineering scaffolds. Gelatin from different sources yields hydrogels with distinctive physical, chemical, and biological properties. In this work, gelatin from bovine skin was used to fabricate hydrogels with varying degrees of crosslinking content using 1, 4, 7, and 10 mL MAA. The material properties of these hydrogels were characterized. The cytocompatibility of the gelatin-based hydrogels was studied using L6 rat myoblasts. The hydrogels from bovine skin gelatin exhibit mechanical properties that are conducive for applications which require substrates to propagate cell growth, migration, and proliferation rapidly. These hydrogels exhibit exceptional tunability behavior which makes them useful and applicable to culture different cell types.
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机译：化学上和结构上模仿天然组织的水凝胶已被广泛用于各种组织工程应用。明胶可以天然地来自不同的来源，并被官能化以制备显示出高细胞相容性和有利的生物可降解特性的水凝胶。明胶主链上的氨基可通过添加不同比例的甲基丙烯酸酐（MAA）来取代，以制成仿生水凝胶，用作组织工程支架。来自不同来源的明胶产生具有独特的物理，化学和生物学特性的水凝胶。在这项工作中，使用1、4、7和10 mL MAA，将牛皮肤明胶用于制备具有不同交联度的水凝胶。表征了这些水凝胶的材料性质。使用L6大鼠成肌细胞研究了基于明胶的水凝胶的细胞相容性。来自牛皮肤明胶的水凝胶显示出机械性能，有利于需要底物快速传播细胞生长，迁移和增殖的应用。这些水凝胶表现出出色的可调性性能，这使其非常有用，可用于培养不同类型的细胞。
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