Localization and synthesis of hyaluronic acid in the cumulus cells and mural granulosa cells of the preovulatory follicle☆

Antonietta Salustri; Masaki Yanagishita; Charles B. Underhill; Torvard C. Laurent; Vincent C. Hascall

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Localization and synthesis of hyaluronic acid in the cumulus cells and mural granulosa cells of the preovulatory follicle☆

机译：透明质酸在排卵前卵泡的卵丘细胞和壁颗粒细胞中的定位和合成☆

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Muralandcumulusgranulosacellssynthesizehyaluronicacid(HA)andexpandinvitroinresponsetofollicle-stimulatinghormoneandasolublefactor(s)producedbyfullygrownoocytes.InthepresentstudyweexaminedHAsynthesisandextracellularmatrixorganizationbythetwocellpopulationsinvivoduringthepreovulatoryperiod.Afterinjectionofhumanchorionicgonadotropinintopregnantmares'serumgonadotropin-primedanimals,aprogressiveincreaseinHAsynthesiswasobservedbythecumuluscell-oocytecomplex(COC),andbythemuralgranulosacellsadjacenttotheantrum(antralgranulosacells).Theoutermostlayersofmuralgranulosacells(peripheralgranulosacells)didnotsynthesizeHA.NetHAsynthesiswasa??4pg/cellforCOCsisolatedafterfullexpansioninducedeitherinvivoorinvitro,whereasthetotalHAcontentandcellnumberintheovulatedCOC(a??11ngHAanda??3000cellsperCOC)wereaboutthreefoldhigherthanforCOCsexpandedinvitro(a??4ngHAanda??1000cellsperCOC).TheincreasedcellcontentofovulatedCOCsappearstobeprimarilytheresultofinclusionofproximalmuralgranulosacellswhichsynthesizeHAinresponsetotheoocytefactor(s)andbecomeincorporatedintheexpandedCOCextracellularmatrixmass.Mediaconditionedbyoocytesenclosedinthecumuluscellmass(intactCOCs)containedonly10a€“20%oftheHA-stimulatoryactivityofmediaconditionedbyanequalnumberofisolatedoocyteswhentestedonmuralgranulosacellcultures.Further,HA-stimulatoryactivityofmediaconditionedbyisolatedoocyteswasdramaticallyreduced(a??70%)bypreincubationfor5hrwithcumuluscellscomparedtopreincubationintheabsenceofcells.TheresultssuggestthatdifferencesinHAsynthesisbetweensubregionsofmembranagranulosadependonadiffusiongradientoftheoocytefactor(s).

机译：Muralandcumulusgranulosacellssynthesizehyaluronicacid（HA）andexpandinvitroinresponsetofollicle-stimulatinghormoneandasolublefactor（S）producedbyfullygrownoocytes.InthepresentstudyweexaminedHAsynthesisandextracellularmatrixorganizationbythetwocellpopulationsinvivoduringthepreovulatoryperiod.Afterinjectionofhumanchorionicgonadotropinintopregnantmares＆＃X27; serumgonadotropin-primedanimals，aprogressiveincreaseinHAsynthesiswasobservedbythecumuluscell-oocytecomplex（COC），andbythemuralgranulosacellsadjacenttotheantrum（antralgranulosacells）.Theoutermostlayersofmuralgranulosacells（peripheralgranulosacells）didnotsynthesizeHA.NetHAsynthesiswasa ?? 4PG / cellforCOCsisolatedafterfullexpansioninducedeitherinvivoorinvitro，whereasthetotalHAcontentandcellnumberintheovulatedCOC（一11ngHAanda？3000个细胞/ COC的数量比体外扩增的COC高4倍（？4ngHAanda？1000个细胞/ COC）。排卵的COC的细胞含量的增加主要表现为以麻黄素形式合成的近端细小嗜藻细胞。 nresponsetotheoocytefactor（S）andbecomeincorporatedintheexpandedCOCextracellularmatrixmass.Mediaconditionedbyoocytesenclosedinthecumuluscellmass（intactCOCs）containedonly10a€“20％oftheHA-stimulatoryactivityofmediaconditionedbyanequalnumberofisolatedoocyteswhentestedonmuralgranulosacellcultures.Further，HA-stimulatoryactivityofmediaconditionedbyisolatedoocyteswasdramaticallyreduced（一个?? 70％）bypreincubationfor5hrwithcumuluscellscomparedtopreincubationintheabsenceofcells.TheresultssuggestthatdifferencesinHAsynthesisbetweensubregionsofmembranagranulosadependonadiffusiongradientoftheoocytefactor（一个或多个）。

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《Developmental biology》 |1992年第2期|共页
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Antonietta Salustri; Masaki Yanagishita; Charles B. Underhill; Torvard C. Laurent; Vincent C. Hascall;
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机译：促分裂原激活蛋白激酶参与促黄体激素诱导的小鼠排卵前毛囊壁和积颗粒细胞中类固醇生成和类固醇生成基因表达的差异调节。
2. LH-Receptor Gene Expression in Human Granulosa and Cumulus Cells from Antral and Preovulatory Follicles [J] . Janni Vikkels? Jeppesen, Stine Gry Kristensen, Maria Eils? Nielsen, The journal of clinical endocrinology and metabolism . 2012,第8期

机译：LH受体基因表达在人的颗粒和窦房和排卵前卵泡细胞中。
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6. LH-Receptor Gene Expression in Human Granulosa and Cumulus Cells from Antral and Preovulatory Follicles [O] . Janni Vikkelsø Jeppesen, Stine Gry Kristensen, Maria Eilsø Nielsen, -1

机译：LH受体基因表达在人的颗粒和窦房和排卵前卵泡细胞中。
7. Participation of Mitogen-activated Protein Kinase in Luteinizing Hormone-induced Differential Regulation of Steroidogenesis and Steroidogenic Gene Expression in Mural and Cumulus Granulosa Cells of Mouse Preovulatory Follicles. [O] . Su, You-Qiang, Nyegaard, Mette, Overgaard, Michael Toft, 2006

机译：丝裂素活化蛋白激酶参与促黄体激素诱导的小鼠排卵前卵泡壁细胞和卵泡颗粒细胞中类固醇生成和类固醇基因表达的差异调节。

Localization and synthesis of hyaluronic acid in the cumulus cells and mural granulosa cells of the preovulatory follicle☆

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