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首页> 外文期刊>Journal of Clinical Microbiology >A study of possible biohazards in the fluorescent antibody test using adenovirus, coxsackievirus, herpesvirus, and respiratory syncytial virus as antigens.
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A study of possible biohazards in the fluorescent antibody test using adenovirus, coxsackievirus, herpesvirus, and respiratory syncytial virus as antigens.

机译：使用腺病毒，柯萨奇病毒，疱疹病毒和呼吸道合胞病毒作为抗原的荧光抗体测试中可能的生物危害性研究。

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摘要

Infectious adenovirus type 5 and coxsackievirus type B5, both nonlipid-containing viruses, were isolated from cells fixed in acetone at 22 degrees C for 15 min, from acetone used for fixation, from the solution used for washing slides during the fluorescent antibody procedure, and after complete processing of antigen preparations with serial twofold dilutions of human antisera and fluorescein-labeled goat anti-human immunoglobulin G. Lipid-containing herpes simplex virus type 1 and respiratory syncytial virus were inactivated by acetone, and infectious virus could not be recovered at any stage in the fluorescent antibody test. Fixation in acetone at 56 degrees C destroyed the infectivity of adenovirus 5 and coxsackievirus B5 within 30 min, but no adverse effect on the antigenic determinants of either virus occurred until after 60 min, thus demonstrating that these antigens can be utilized without the hazard of infectious virus.
机译：从22°C固定在丙酮中固定15分钟的细胞，固定用的丙酮，荧光抗体程序中用于洗涤载玻片的溶液中分离出5种传染性腺病毒和B5型柯萨奇病毒，它们均不含脂质。在将人抗血清和荧光素标记的山羊抗人免疫球蛋白G连续两次稀释的抗原制剂完全加工后，丙酮将灭活了含脂质的1型单纯疱疹病毒和呼吸道合胞病毒，并且在任何情况下都无法回收感染性病毒。在荧光抗体测试阶段。在56°C的丙酮中固定可在30分钟内破坏腺病毒5和柯萨奇病毒B5的传染性，但直到60分钟后才对这两种病毒的抗原决定簇产生不利影响，因此证明这些抗原可以被利用而没有感染的危险。病毒。

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