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首页> 外文期刊>Journal of Clinical Microbiology >Modified coagglutination procedure for the serological grouping of streptococci.
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Modified coagglutination procedure for the serological grouping of streptococci.

机译：改进的凝集程序，用于链球菌的血清学分组。

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Cowan I staphylococci coated with antisera to streptococcal groups A, B, C, D, F, and G were used as coagglutination reagents in a modified coagglutination procedure (MCAP). Streptococcal group antigens were extracted with a Streptomyces albus-lysozyme enzyme mixture for 30 min at 55 degrees C and centrifuged, and the supernatant was tested by slide coagglutination. Positive coagglutination reactions occurred within 30 s. The cell pellets from overnight broth cultures and colonies taken directly from sheep blood agar plates were tested and compared with the results of the Lancefield capillary precipitin method. Of the 102 strains of broth-grown cells tested, 100 were grouped by the MCAP and the Lancefield capillary precipitin method. The remaining two isolates were serologically identified only by the MCAP. Of the original 102 strains, 97 were tested by MCAP after extraction of five well-isolated colonies from a sheep blood agar plate. When this latter method was used, 95.9% of the strains were correctly identified. Nonspecific reactions were observed only while testing the MCAP with the direct plate assay. These cross-reactions were remedied promptly by either absorption or dilution of the antisera involved. The MCAP was found to be a rapid and reliable technique for the serological grouping of streptococci.
机译：在改良的凝集程序（MCAP）中，将抗血清包被的链球菌A，B，C，D，F和G的Cowan I葡萄球菌用作凝集试剂。链球菌群抗原用链霉菌溶菌酶混合物在55摄氏度下提取30分钟并离心，并通过载玻片凝集测试上清液。阳性凝集反应在30 s内发生。测试了过夜肉汤培养物中的细胞沉淀和直接取自羊血琼脂平板的菌落，并将其与Lancefield毛细管沉淀法的结果进行了比较。在测试的102个肉汤生长细胞菌株中，通过MCAP和Lancefield毛细管沉淀法将100个菌株分组。其余两个分离株仅通过MCAP在血清学上鉴定。从绵羊血琼脂平板中提取五个完全分离的菌落后，在最初的102个菌株中，有97个通过MCAP检测。使用后一种方法时，正确鉴定出了95.9％的菌株。仅在使用直接平板测定法测试MCAP时观察到非特异性反应。通过吸收或稀释所涉及的抗血清可以迅速纠正这些交叉反应。已发现MCAP是链球菌血清学分组的一种快速而可靠的技术。

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