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首页> 外文期刊>Journal of Clinical Microbiology >Simplified coagglutination test for serological grouping of beta-hemolytic streptococci.
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Simplified coagglutination test for serological grouping of beta-hemolytic streptococci.

机译：β-溶血性链球菌血清学分组的简化凝集试验。

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A new coagglutination kit consisting of a plastic slide with dried antibody-coated staphylococci was evaluated for the grouping of beta-hemolytic streptococci of groups A, B, C, and G. The test was compared with the classical precipitation test, using hot formamide antigen extracts with 224 strains of groups A, B, C, and G streptococci. An agreement of 100% was found between the new coagglutination and the classical precipitation procedures. No false-positive results were obtained with group F and D streptococci; however, group L streptococci reacted with the group A reagent. The test procedure could be shortened by using suspensions of colonies from overnight cultures on blood agar plates in small volumes of Todd-Hewitt broth without further incubation. All 74 strains of groups A, B, C, and G were correctly identified from suspensions in Todd-Hewitt broth; however, suspending the colonies in 0.9% saline or phosphate-buffered saline resulted in lower sensitivities.
机译：对由塑料载玻片和干燥的抗体包被的葡萄球菌组成的新的凝集试剂盒，对A，B，C和G组的β溶血性链球菌进行了分组评估。使用热甲酰胺抗原将该试验与经典沉淀试验进行了比较提取物具有224个A，B，C和G组链球菌菌株。在新的凝集法和经典的沉淀程序之间发现100％的一致性。 F和D组链球菌均未获得假阳性结果。然而，L组链球菌与A组试剂反应。通过在少量琼脂肉汤中使用血琼脂平板上过夜培养的菌落悬浮液可以缩短测试程序，而无需进一步孵育。从Todd-Hewitt肉汤中的悬浮液正确鉴定出A，B，C和G组的所有74株菌株。但是，将菌落悬浮在0.9％的盐水或磷酸盐缓冲液中会导致敏感性降低。

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