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首页> 外文期刊>Journal of Clinical Microbiology >Comparison of various McCoy cell treatment procedures used for detection of Chlamydia trachomatis.
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Comparison of various McCoy cell treatment procedures used for detection of Chlamydia trachomatis.

机译：用于检测沙眼衣原体的各种McCoy细胞处理程序的比较。

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McCoy cells treated in six different ways, in addition to untreated cells, were compared to determine which gave rise to the largest number of Chlamydia trachomatis inclusions when tested with a laboratory-passaged strain. The same batch of cells was treated by irradiation, preinoculation exposure to cytochalasin B or 5-iodo-2'-deoxyuridine, and postinoculation exposure to cycloheximide, hydrocortisone, or emetine. Significantly more inclusions were always found in cells which had been treated with cycloheximide than in cells treated in any other way. Conversely, untreated McCoy cells always had significantly fewer inclusions than cells which had received some form of treatment. Similar results were obtained when cycloheximide-treated, irradiated, and untreated cells were inoculated with urethral specimens containing unpassaged organisms.
机译：除了用未经处理的细胞外，还比较了用六种不同方法处理过的McCoy细胞，以确定当用实验室传代的菌株进行测试时，它们会导致最大数量的沙眼衣原体包涵体的产生。通过辐射，接种前暴露于细胞松弛素B或5-碘-2'-脱氧尿苷，以及接种后暴露于环己酰亚胺，氢化可的松或曲美汀来处理同一批次的细胞。与用其他方法处理过的细胞相比，用环己酰亚胺处理过的细胞中总发现有更多的内含物。相反，未经处理的McCoy细胞的夹杂物总是比接受某种处理的细胞少得多。当用含有未传代生物的尿道标本接种环己酰亚胺处理，辐照和未处理的细胞时，可获得相似的结果。

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