...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Clinical Microbiology >Direct micromethod for diagnosis of acute and congenital Chagas' disease.
【24h】

Direct micromethod for diagnosis of acute and congenital Chagas' disease.

机译：用于诊断急性和先天性恰加斯病的直接方法。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A microhematocrit concentration method (MH) for immediate diagnosis of Chagas' disease during the acute stage or in congenital cases was standardized. Parasitemia as low as 1,000 parasites per ml was detected, after centrifugation of six 50-microliters capillary tubes, by 10-min microscopic observation of each buffy coat spread between slide and cover glass. Operator's time was reduced by at least one-third when compared with a fresh blood observation (FB). In 12 of the 15 patients studied, diagnosis was performed in 4.9 +/- 3.08 min with MH, whereas 27.0 +/- 12.1 min were necessary when FB was used. In the three remaining patients whose FB results were negative, MH became positive after 13, 16, and 40 min. In our experience, FB proved to be more sensitive than previously reported. Suckling mouse inoculation also proved to be sensitive but, as in xenodiagnosis and in hemoculture, the delay in getting the final result was a limiting factor.
机译：标准化了用于在急性期或先天性病例中立即诊断恰加斯氏病的微血细胞比容浓缩方法（MH）。在将六个50微升毛细管离心后，通过对玻片和盖玻片之间铺满的每块血沉棕黄层进行10分钟显微镜观察，检出的寄生虫病低至每毫升1,000寄生虫。与新鲜血液观察（FB）相比，操作员的时间至少减少了三分之一。在研究的15位患者中，有12位在MH的诊断时间为4.9 +/- 3.08分钟，而使用FB则需要27.0 +/- 12.1分钟。在剩余三名FB结果均为阴性的患者中，MH在13、16和40分钟后变为阳性。根据我们的经验，FB被证明比以前报道的更为敏感。哺乳小鼠接种也被证明是敏感的，但是，正如在异种诊断和血液培养中一样，获得最终结果的延迟是一个限制因素。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号