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首页> 外文期刊>Journal of Clinical Microbiology >Rapid identification of Clostridium difficile by toxin detection.
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Rapid identification of Clostridium difficile by toxin detection.

机译:通过毒素检测快速鉴定艰难梭菌。

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Rapid identification of Clostridium difficile in a stool specimen could be accomplished within 24 h by detection of toxin elaborated in an agar or broth culture containing cycloserine and cefoxitin. Broth culture seemed to give a more rapid and sensitive result than the agar plate culture. For cultivation of C. difficile in stool, we recommend the use of chopped meat broth and blood agar plate, the former for toxin detection in 1 to 2 days and the latter for colonial morphology and isolation of a pure culture.
机译:通过检测在含有环丝氨酸和头孢西丁的琼脂或肉汤培养物中精心制作的毒素,可以在24小时内快速鉴定粪便样品中的艰难梭菌。肉汤培养似乎比琼脂平板培养更快,更灵敏。为了在粪便中培养艰难梭菌,我们建议使用切碎的肉汤和血琼脂平板,前者用于1至2天的毒素检测,后者用于殖民地形态和纯培养物的分离。

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