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首页> 外文期刊>Journal of Clinical Microbiology >Production of leukocidin by clinical isolates of Pseudomonas aeruginosa and antileukocidin antibody from sera of patients with diffuse panbronchiolitis.
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Production of leukocidin by clinical isolates of Pseudomonas aeruginosa and antileukocidin antibody from sera of patients with diffuse panbronchiolitis.

机译：铜绿假单胞菌临床分离株和抗纤毛蛋白抗体从弥漫性全细支气管炎患者血清中产生白细胞介素。

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The ratio of leukocidin-producing strains to clinical isolates of Pseudomonas aeruginosa was investigated together with the production of protease, elastase, and exotoxin A. We also examined whether these strains contain the common antigen which resides in the cell wall. By using the agar gel diffusion test with specific antisera, we found that 87 of 90 (96.7%) of clinical isolates produced leukocidin. Protease, elastase, and exotoxin A were also produced at high percentages. The common antigen was found to exist in all strains. Next, to estimate antileukocidin antibody in the sera of patients, we used an enzyme-linked immunosorbent assay with horseradish peroxidase-protein A. The sera of 39 patients with diffuse panbronchiolitis (DPB) were investigated for antileukocidin antibody. The mean antileukocidin titer in the sera of 17 DPB patients who were not infected with P. aeruginosa and 5 DPB patients who were transiently infected with the bacteria was about the same as the mean antileukocidin titer in the sera of 11 healthy controls, whereas the mean antileukocidin titer in the sera of 17 DPB patients who were persistently colonized was significantly higher than that in healthy controls. These results indicate that leukocidin was produced at the local site of infection in DPB patients.
机译：研究了白细胞介素生产菌株与铜绿假单胞菌临床分离株的比率，以及蛋白酶，弹性蛋白酶和外毒素A的产生。我们还检查了这些菌株是否含有驻留在细胞壁中的共同抗原。通过将琼脂凝胶扩散试验与特定抗血清结合使用，我们发现90株临床分离物中的87株（96.7％）产生了白细胞抑制素。蛋白酶，弹性蛋白酶和外毒素A的含量也很高。发现所有菌株中都存在共同抗原。接下来，为了评估患者血清中的抗胆红素抗体，我们使用了辣根过氧化物酶蛋白A的酶联免疫吸附试验。对39例弥漫性全细支气管炎（DPB）患者的血清中抗胆红素抗体进行了研究。未感染铜绿假单胞菌的17例DPB患者和短暂感染细菌的5例DPB患者的血清中平均抗胆红素滴度与11名健康对照者血清中的平均抗胆红素滴度大致相同，而平均值持续定殖的17例DPB患者血清中的抗胆红素滴度明显高于健康对照者。这些结果表明在DPB患者中在感染的局部位点产生白细胞抑制素。

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