Inadequate mixing in microdilution wells during preparation of antimicrobial dilutions for susceptibility testing.

J G Zhi; C H Nightingale; R Quintiliani

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Inadequate mixing in microdilution wells during preparation of antimicrobial dilutions for susceptibility testing.

机译：在制备用于药敏试验的抗菌稀释液时，微量稀释孔中的混合不足。

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An equation representing a twofold series of antibiotic dilutions was derived. The theoretical results were then validated with experimental observations of piperacillin against Pseudomonas aeruginosa. Insufficient mixing leads to an erroneously low MIC. It is concluded that maintaining antibiotic concentration equilibrium during microdilution is required for accurate microdilution susceptibility tests.

机译：得出了代表抗生素稀释度的两倍系列的方程式。然后用哌拉西林对铜绿假单胞菌的实验观察结果验证了理论结果。混合不充分会导致错误的低MIC。结论是，进行精确的微量稀释药敏试验需要在微量稀释期间保持抗生素浓度平衡。

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《Journal of Clinical Microbiology》 |1988年第10期|共3页
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J G Zhi; C H Nightingale; R Quintiliani;
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中图分类医学微生物学（病原细菌学、病原微生物学）;
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2. Comparison of agar dilution, broth microdilution, disk diffusion, E-test, and BACTEC radiometric methods for antimicrobial susceptibility testing of clinical isolates of the Nocardia asteroides complex. [J] . A Ambaye, P C Kohner, P C Wollan, Journal of Clinical Microbiology . 1997,第4期

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3. Comparative evaluation of the E test, agar dilution, and broth microdilution for testing susceptibilities of Helicobacter pylori strains to 20 antimicrobial agents. [J] . R Piccolomini, G Di Bonaventura, G Catamo, Journal of Clinical Microbiology . 1997,第7期

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4. EVALUATION OF THE ANTIMICROBIAL RESISTANCE OF S. AUREUS AND STREPTOCOCCI ISOLATES WITH ANTIMICROBIAL DISK AND DILUTION SUSCEPTIBILITY TEST [C] . G. Koster, B.A. Tenhagen, A. Hille, Annual Meeting of the National Mastitis Council . 2004

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6. Inadequate mixing in microdilution wells during preparation of antimicrobial dilutions for susceptibility testing. [O] . J G Zhi, C H Nightingale, R Quintiliani 1988

机译：在制备用于药敏试验的抗菌稀释液时微量稀释孔中的混合不足。
7. Inadequate mixing in microdilution wells during preparation of antimicrobial dilutions for susceptibility testing [O] . J G Zhi, C H Nightingale, R Quintiliani 1988

机译：在制备抗菌稀释度的微量稀释井中的微量稀释井中的混合不足
8. Carbapenem Susceptibility Testing Errors Using Three Automated Systems, Disk Diffusion, Etest, and Broth Microdilution and Carbapenem Resistance Genes in Isolates of Acinetobacter baumannii-calcoaceticus Complex. [R] . Markelz, A. E., Mende, K., Murray, C. K., 2011

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Inadequate mixing in microdilution wells during preparation of antimicrobial dilutions for susceptibility testing.

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