...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Clinical Microbiology >Novel densitometric method for endonuclease analysis of Escherichia coli DNA samples containing multiple plasmids.
【24h】

Novel densitometric method for endonuclease analysis of Escherichia coli DNA samples containing multiple plasmids.

机译：新型光密度测定法，用于分析含有多个质粒的大肠杆菌DNA样品的核酸内切酶。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We developed a novel method whereby the digestion pattern of each plasmid was distinguished in a sample of endonuclease-digested plasmid DNAs which contained multiple plasmids extracted from a bacterial strain. This method consisted of two procedures. (i) The concentration ratio of each undigested plasmid DNA and of each digested DNA fragment was calculated on the basis of densitometric scanning of an electrophoretogram, and the concentration ratio was then compared with the theoretical concentration ratio to determine from which plasmid each fragment was generated. (ii) The second procedure involved rapid visual identification with a scanning graph. We thus analyzed five strains of Escherichia coli that harbored several cryptic plasmids. The two procedures made it possible to analyze the restriction digestion pattern of each plasmid without the need to isolate the individual plasmids, except in the case of certain fragments. Even when the digested patterns of these exceptional fragments could not be distinguished completely, however, our method had the advantage that peak patterns of plasmids could be compared visually among different bacterial strains.
机译：我们开发了一种新方法，可在核酸内切酶消化的质粒DNA样品中区分每个质粒的消化模式，该DNA包含从细菌菌株中提取的多个质粒。该方法由两个过程组成。 （i）基于电泳图的光密度扫描计算每个未消化的质粒DNA和每个消化的DNA片段的浓度比，然后将该浓度比与理论浓度比相比较以确定每个片段是从哪个质粒产生的。 （ii）第二种方法包括使用扫描图快速视觉识别。因此，我们分析了具有几种隐性质粒的五种大肠杆菌菌株。这两种方法使得可以分析每个质粒的限制性消化模式，而无需分离单个质粒，除非是某些片段。但是，即使无法完全区分这些例外片段的消化模式，我们的方法仍具有可以在不同细菌菌株之间目视比较质粒峰模式的优势。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号