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首页> 外文期刊>Journal of Clinical Microbiology >Unusual Enterobacteriaceae: lactose-positive Salmonella typhimurium which is endemic in S?o Paulo, Brazil.
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Unusual Enterobacteriaceae: lactose-positive Salmonella typhimurium which is endemic in S?o Paulo, Brazil.

机译:不寻常的肠杆菌科:乳糖阳性的鼠伤寒沙门氏菌是巴西圣保罗的地方病。

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Since 1971 a lactose-positive (lac+) Salmonella typhimurium variety Copenhagen has been endemic in the city of Sao Paulo. The strain is a strong lactose fermenter and resembles Escherichia coli on primary plating media and in triple sugar iron agar. Although most isolates of the strain have uniform properties, some have slightly different antigens, antibiograms, phage types, or fermentation patterns. Most isolates have come from stools of infants under 1 year of age and are probably hospital acquired; however, other isolates are probably community acquired. Eighteen other lac+ Salmonella isolated in the United States were also studied. Most of these strains resembled E. coli on primary plates and triple sugar iron agar; thus their identification would pose a problem for most clinical laboratories. A simple procedure for detecting lac+ Salmonella mixed with lac+ E. coli consists of touching 12 colonies in succession with a straight wire and then inoculating a peptone iron agar tube. H2S production is apparent from lac+ Salmonella even if 11 E. coli and one Salmonella colony are picked. If a positive peptone iron agar tube is observed, then individual colonies are tested to rule out other strong H2S producers. The true incidence of lac+ Salmonella is unknown because they are not isolated and identified in most laboratories.
机译:自1971年以来,乳糖阳性(lac +)鼠伤寒沙门氏菌变种哥本哈根已经在圣保罗市流行。该菌株是一种强力的乳糖发酵罐,在主要的平板培养基和三糖铁琼脂中类似于大肠杆菌。尽管该菌株的大多数分离株具有统一的特性,但有些分离株的抗原,抗菌谱,噬菌体类型或发酵模式略有不同。大多数分离株来自1岁以下婴儿的粪便,很可能是医院获得的。但是,其他隔离株可能是社区获得的。还研究了在美国分离的其他十八种lac +沙门氏菌。这些菌株中的大多数类似于初生平板和三糖铁琼脂上的大肠杆菌。因此,对大多数临床实验室而言,它们的识别将带来问题。检测lac +沙门氏菌与lac +大肠杆菌混合的简单方法是,用直的线连续接触12个菌落,然后接种蛋白ept琼脂管。即使选择了11个大肠杆菌和一个沙门氏菌菌落,lac +沙门氏菌仍会产生H2S。如果观察到蛋白p铁琼脂管呈阳性,则测试单个菌落以排除其他强大的H2S生产者。 lac +沙门氏菌的真正发病率尚不清楚,因为在大多数实验室中并未分离和鉴定出它们。

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