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首页> 外文期刊>Journal of Clinical Microbiology >Detection of heat-labile-enterotoxin-producing colonies of Escherichia coli and Vibrio cholerae by solid-phase sandwich radioimmunoassays.
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Detection of heat-labile-enterotoxin-producing colonies of Escherichia coli and Vibrio cholerae by solid-phase sandwich radioimmunoassays.

机译：固相夹心放射免疫分析法检测大肠杆菌和霍乱弧菌产热不稳定肠毒素的菌落。

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摘要

A solid-phase sandwich assay that was able to differentiate heat-labile-enterotoxin-producing colonies of Escherichia coli and choleratoxin-producing colonies of Vibrio cholerae from nontoxigenic colonies is described. Flexible polyvinyl chloride plastic film coated with antibody molecules was allowed to react with partially lysed bacterial colonies in a standard petri dish. The immobilized antigen on the plastic film was then labeled with radioiodinated antibody. Autoradiography identified antigen-containing colonies. As little as 5 to 25 pg of pure toxin contained in a 3- to 4-mm-diameter circle was reliably detected by this method. The synthesis of heat-labile enterotoxin and choleratoxin by cells growing on selective media such as eosin methylene blue agar, MacConkey agar, Endo agar, and thiosulfate-citrate-bile salts-sucrose agar was demonstrated. The method appears to be suitable for large-scale surveys.
机译：描述了一种能够将产生热不稳定肠毒素的大肠杆菌菌落和产生霍乱弧菌的霍乱弧菌菌落与无毒菌落菌落区分开的固相夹心法。使涂有抗体分子的柔性聚氯乙烯塑料薄膜与标准培养皿中的部分裂解的细菌菌落反应。然后用放射性碘标记的抗体标记塑料膜上固定的抗原。放射自显影确定了含抗原的菌落。通过这种方法，可以可靠地检测出直径3至4毫米的圆中仅含5至25 pg的纯毒素。证明了通过在诸如曙红亚甲基蓝琼脂，MacConkey琼脂，Endo琼脂和硫代硫酸盐-柠檬酸盐-胆汁盐-蔗糖琼脂等选择性培养基上生长的细胞合成热不稳定的肠毒素和胆汁毒素。该方法似乎适合于大规模调查。

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