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首页> 外文期刊>Journal of Clinical Microbiology >Serological evaluation of Escherichia coli-expressed human T-cell leukemia virus type I env, gag p24, and tax proteins.
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Serological evaluation of Escherichia coli-expressed human T-cell leukemia virus type I env, gag p24, and tax proteins.

机译：大肠杆菌表达的人类I型en T细胞白血病病毒env，gag p24和tax蛋白的血清学评估。

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摘要

Three proteins (env, gag, and tax) encoded by the human T-cell leukemia virus type I (HTLV-I) genome were cloned and expressed in Escherichia coli. The env protein contained a substantial part of the gp46 domain and a majority of the p21e domain. The gag protein contained all of p24 and portions of p19 and p15. In addition to these two structural proteins, a full-length tax (p40X) construct was obtained. All three recombinant proteins were purified to near homogeneity. When used in an immunoblot assay, the three recombinant proteins detected antibodies in more HTLV-I antibody-positive patient sera than did the corresponding native proteins. Antibodies to at least two of these three different gene products were detected in 98.4% of adult T-cell leukemia patients, 100% of HTLV-I-associated myelopathy patients, 97.4% of asymptomatic carriers, and 94% of uncharacterized HTLV-I-positive patients. Antibody to recombinant tax was found in 4.9% of adult T-cell leukemia patients, whereas antibody to recombinant env could not be detected. These recombinant proteins from three different gene products may be useful in detecting or confirming the presence of antibodies to HTLV-I.
机译：克隆了由人类I型T细胞白血病病毒（HTLV-1）基因组编码的三种蛋白质（env，gag和tax），并在大肠杆菌中表达。 env蛋白包含gp46域的大部分和p21e域的大部分。 gag蛋白包含p24的全部以及p19和p15的一部分。除了这两个结构蛋白外，还获得了全长税收（p40X）构建体。所有三种重组蛋白均纯化至接近均一。当用于免疫印迹测定时，这三种重组蛋白比相应的天然蛋白在更多的HTLV-1抗体阳性患者血清中检测到抗体。在98.4％的成人T细胞白血病患者，100％的与HTLV-I相关的脊髓病患者，97.4％的无症状携带者和94％的未鉴定的HTLV-I-患者中，检测到了这三种不同基因产物中至少两种的抗体。阳性患者。在成人T细胞白血病患者中，有4.9％的人发现了重组税抗体，而重组env的抗体无法检测到。来自三种不同基因产物的这些重组蛋白可用于检测或确认针对HTLV-1的抗体的存在。

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