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首页> 外文期刊>Journal of Clinical Microbiology >Several membrane polypeptides of the live vaccine strain Francisella tularensis LVS stimulate T cells from naturally infected individuals.
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Several membrane polypeptides of the live vaccine strain Francisella tularensis LVS stimulate T cells from naturally infected individuals.

机译：活疫苗菌株弗朗西斯菌TVS的几种膜多肽刺激自然感染者的T细胞。

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The currently used live vaccine strain Francisella tularensis LVS was derived several decades ago from a wild strain of the species. In the present report, several membrane polypeptides of LVS are shown to be recognized by T cells from individuals immunized by natural infection with F. tularensis. Bacterial membranes of a capsule-deficient mutant of LVS were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Thereafter, gels were divided into seven fractions, each fraction containing a different number of peptide bands. From other gels, four bands were excised, each containing one major polypeptide. Eluates of each fraction and of each polypeptide band induced a proliferative response and an interleukin-2 response in lymphocytes from most of the individuals. When the lymphocytes were separated after induction, most of the proliferative response was found to occur in CD4+ T cells. Lymphocytes from nonimmune individuals responded poorly to all membrane polypeptides. To study the possible heterogeneity of antigen determinants among the polypeptides, T-cell clones were raised towards F. tularensis and tested for proliferative response to the four major membrane polypeptides. Five clones, all CD4+ CD8-, responded to one or more of the polypeptides, each clone with a unique pattern of response. In conclusion, F. tularensis possesses a high number of T-cell-reactive membrane polypeptides. There seems to be a heterogeneity of T-cell determinants among these polypeptides. Determinants involved in immunization by natural infection are well conserved in LVS.
机译：当前使用的活疫苗菌株弗朗西斯菌tularensis LVS是几十年前从该物种的野生菌株衍生而来。在本报告中，LVS的几种膜多肽显示出被T.ularularensis自然感染免疫的个体的T细胞识别。 LVS的胶囊缺乏突变体的细菌膜进行十二烷基硫酸钠-聚丙烯酰胺凝胶电泳。此后，将凝胶分为七个部分，每个部分包含不同数量的肽带。从其他凝胶中切下四个带，每个带包含一个主要多肽。每个部分和每个多肽带的洗脱液在大多数个体的淋巴细胞中诱导了增殖反应和白介素2反应。诱导后分离淋巴细胞时，发现大多数增殖反应发生在CD4 + T细胞中。来自非免疫个体的淋巴细胞对所有膜多肽的反应较差。为了研究多肽中抗原决定簇的可能异质性，将T细胞克隆引向土拉弗朗西斯菌，并测试其对四种主要膜多肽的增殖反应。所有CD4 + CD8-的五个克隆对一种或多种多肽有反应，每个克隆都有独特的反应模式。总之，土拉弗朗西斯菌具有大量的T细胞反应性膜多肽。这些多肽之间似乎存在T细胞决定簇的异质性。通过自然感染进行免疫的决定簇在LVS中保存良好。

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