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首页> 外文期刊>Journal of Clinical Microbiology >Comparison of the API 20E and Oxi/Ferm systems in identification of nonfermentative and oxidase-positive fermentative bacteria.
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Comparison of the API 20E and Oxi/Ferm systems in identification of nonfermentative and oxidase-positive fermentative bacteria.

机译：API 20E和Oxi / Ferm系统在非发酵和氧化酶阳性发酵菌鉴定中的比较。

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The API 20E and Oxi/Ferm systems were tested in parallel to identify nonfermentative bacteria and oxidase-positive fermentative bacteria. Test strains consisted of consecutive clinical isolates, with stock cultures used to supplement those species infrequently recovered. The two microsystems, as well as tubes of triple sugar iron, motility, cetrimide, and oxidative glucose media, were inoculated by each worker for each organism. Identification of each isolate was by the protocol of the manufacturers, with supplemental tests and flagella stains performed when necessary. Concurrent identification was undertaken with a conventional system against which the results of the two systems were compared for accuracy. There was a 95.3% accuracy in identification by the Oxi-Ferm system and 88.9% by the API system. Almost one-fourth of all identification attempts with the API required computer assistance, and most of these were for oxidase positive bacteria. Because of this, and because the API system showed greater accuracy in identification of the oxidase-negative bacteria, it seems best suited for identification of these organisms (P. maltophilia, A. anitratus, and A. lwoffi). The Oxi/Ferm system is technically less cumbersome than the API and is well suited for both groups of organisms.
机译：对API 20E和Oxi / Ferm系统进行了并行测试，以鉴定非发酵菌和氧化酶阳性发酵菌。测试菌株由连续的临床分离株组成，用原种培养物补充不常回收的物种。每个工人都为每种生物接种了两个微系统，以及三重糖铁，运动性，西曲肽和氧化性葡萄糖培养基的试管。根据制造商的方案鉴定每种分离物，必要时进行补充测试和鞭毛染色。使用常规系统进行并发识别，将其与两个系统的结果进行比较以确保准确性。 Oxi-Ferm系统的识别准确度为95.3％，API系统的识别准确度为88.9％。使用API​​进行的所有识别尝试中，几乎有四分之一需要计算机协助，其中大多数是针对氧化酶阳性细菌的。因此，并且因为API系统在识别氧化酶阴性细菌方面显示出更高的准确性，所以它似乎最适合于识别这些生物（嗜麦芽孢杆菌，苯胺曲霉和lwoffi菌）。从技术上讲，Oxi / Ferm系统比API麻烦，并且非常适合两组生物。

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