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首页> 外文期刊>Journal of Clinical Microbiology >Bacteriocins as tools in analysis of nosocomial Klebsiella pneumoniae infections.
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Bacteriocins as tools in analysis of nosocomial Klebsiella pneumoniae infections.

机译：细菌素作为分析医院内肺炎克雷伯菌感染的工具。

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摘要

Epidemiological analysis of isolates from nosocomial infections caused by Klebsiella pneumoniae was improved by the use of bacteriocins in addition to capsular serotyping. Screening for bacteriocins produced by 77 reference strains for capsular serotyping identified 39 strains, and 8 of these strains were selected as a typing set. Using this set, we found that 241 to 259 (91%) nonepidemic clinical isolates of K. pneumoniae were inhibited by one or more of the eight producers. Of the most frequent bacteriocin type there were 31 examples (12%). High reproducibility of typing patterns (83.3%) and easy practicability of typing were achieved with a streak-and-point method avoiding the use of suspensions of bacteriocins and the risk of instability. The Klebsiella bacteriocins were active also on Enterobacter and Shigella species and on Escherichia coli strains, but were ineffective on other Enterobacteriacae.
机译：除荚膜血清分型外，还通过使用细菌素改善了由肺炎克雷伯菌引起的医院感染分离株的流行病学分析。筛选由荚膜血清分型的77株参考菌株产生的细菌素，鉴定出39株，其中8株被选为分型。使用这组数据，我们发现八个生产者中的一个或多个抑制了241至259个（91％）肺炎克雷伯菌的非流行性临床分离株。在最常见的细菌素类型中，有31例（12％）。使用条纹和点方法可以实现打字模式的高可重复性（83.3％）和易于打字的实用性，避免了使用细菌素悬浮液和不稳定的风险。克雷伯菌细菌素对肠杆菌和志贺氏菌属以及大肠杆菌菌株也有活性，但对其他肠杆菌无效。

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