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首页> 外文期刊>Journal of Clinical Microbiology >Identification of Pseudomonas aeruginosa by pyocyanin production on Tech agar.
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Identification of Pseudomonas aeruginosa by pyocyanin production on Tech agar.

机译：在Tech琼脂上通过绿脓素生产鉴定铜绿假单胞菌。

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Pseudomonas aeruginosa is the only gram-negative bacillus capable of producing the very distinctive water-soluble pigment pyocyanin. We evaluated the reliability of this characteristic as a unique test for the identification of this organism by using Tech agar (BBL Microbiology Systems, Cockeysville, Md.) medium. A retrospective and prospective analysis was performed with a total of 835 strains of P. aeruginosa; 818 (98%) produced pigment within 48 h of incubation, and 96% of those which produced pigment were positive after overnight incubation. Seventeen strains (2.0%) failed to produce pigment; 15 were mucoid strains from patients with cystic fibrosis. Tech agar is an effective, simple, and inexpensive medium for P. aeruginosa identification and may be used as a unique test for all potential P. aeruginosa isolates (beta hemolytic on blood agar; lactose-negative, oxidase-positive colonies). Nonpigmented mucoid strains, as well as other nonpigmented organisms, will require additional testing to ensure proper identification.
机译：铜绿假单胞菌是唯一能够产生非常独特的水溶性色素绿脓素的革兰氏阴性杆菌。我们使用Tech agar（BBL Microbiology Systems，Cockeysville，MD）培养基评估了该特性的可靠性，作为鉴定该生物的独特测试。对总共835株铜绿假单胞菌进行了回顾性和前瞻性分析。 818（98％）在培养48小时内产生色素，过夜培养后96％的产生色素阳性。 17个菌株（2.0％）未能产生色素; 15例来自囊性纤维化患者的粘液样菌株。技术琼脂是一种用于铜绿假单胞菌鉴定的有效，简单且廉价的培养基，可以用作所有潜在铜绿假单胞菌分离株（血琼脂上的β溶血;乳糖阴性，氧化酶阳性菌落）的独特检测方法。未着色的粘液样菌株以及其他未着色的生物将需要进行额外的测试，以确保正确鉴定。

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