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首页> 外文期刊>Journal of Clinical Microbiology >Chemically defined medium for Legionella pneumophila growth.
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Chemically defined medium for Legionella pneumophila growth.

机译：化学定义的培养基，用于军团菌的生长。

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A chemically defined medium containing 18 amino acids, inorganic salts, rhamnose, choline, and ferric pyrophosphate has been developed. The final concentrations of salts and amino acids were modeled after yeast extract. This medium supported the growth of four serogroups of Legionella pneumophila. Growth in shake cultures at 37 degrees C produced a lag time of approximately 5 h and a generation time of 4 h with a maximum growth yield of 10 9 colony-forming units per ml. A soluble brown pigment was observed in the stationary phase of growth. The optimal pH was 6.3. Rhamnose and choline were stimulatory; arginine, serine, threonine, cysteine, valine, and methionine were essential. Supplemental iron was not required to attain maximum growth, but iron deprivation caused an extended lag phase.
机译：已开发出一种化学成分确定的培养基，其中包含18个氨基酸，无机盐，鼠李糖，胆碱和焦磷酸铁。在酵母提取物之后模拟盐和氨基酸的最终浓度。该培养基支持嗜肺军团菌四个血清群的生长。摇瓶培养物在37摄氏度下的生长产生大约5小时的滞后时间，产生时间为4小时，最大生长产量为10 9 9每毫升菌落形成单位。在生长的静止期观察到可溶性棕色颜料。最佳pH为6.3。鼠李糖和胆碱具有刺激性。精氨酸，丝氨酸，苏氨酸，半胱氨酸，缬氨酸和蛋氨酸是必不可少的。不需要补充铁就可以达到最大的生长，但是铁的缺乏会导致延迟期的延长。

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