Enzyme-linked immunosorbent assays based on polyclonal and monoclonal antibodies for rotavirus detection.

G M Beards; A D Campbell; N R Cottrell; J S Peiris; N Rees; R C Sanders; J A Shirley; H C Wood; T H Flewett

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Enzyme-linked immunosorbent assays based on polyclonal and monoclonal antibodies for rotavirus detection.

机译：基于多克隆和单克隆抗体的酶联免疫吸附测定，用于轮状病毒检测。

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We describe two enzyme-linked immunosorbent assays for rotavirus antigen in feces, which were designed to be as sensitive and specific as possible, and easy to use anywhere. Both are indirect methods, using the antibody capture method, but the second assay utilizes a rotavirus group-specific monoclonal "detecting" antibody instead of the hyperimmune polyvalent guinea pig antisera used in the first assay. Both tests were found to be more sensitive than electron microscopy for detecting virus. To develop these tests, solid phase, antiserum production methods, treatment of the test antigen with EDTA, substrate, stability of reagents, and the need for confirmatory "blocking" tests were all examined. The first assay described is that used at present by the World Health Organization for their worldwide diarrheal disease control program.

机译：我们描述了粪便中轮状病毒抗原的两种酶联免疫吸附测定法，这些测定法被设计为尽可能灵敏和特异，并且易于在任何地方使用。两种方法都是使用抗体捕获方法的间接方法，但是第二种方法利用轮状病毒组特异性单克隆“检测”抗体代替第一种方法中使用的超免疫多价豚鼠抗血清。发现这两种测试对于检测病毒都比电子显微镜更敏感。为了开发这些测试，检查了固相，抗血清生产方法，用EDTA处理测试抗原，底物，试剂的稳定性以及确认性“封闭”测试的必要性。描述的第一种测定方法是世界卫生组织目前用于其全球腹泻病控制计划的测定方法。

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《Journal of Clinical Microbiology》 |1984年第2期|共7页
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G M Beards; A D Campbell; N R Cottrell; J S Peiris; N Rees; R C Sanders; J A Shirley; H C Wood; T H Flewett;
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中图分类医学微生物学（病原细菌学、病原微生物学）;
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6. Enzyme-linked immunosorbent assays based on polyclonal and monoclonal antibodies for rotavirus detection. [O] . G M Beards, A D Campbell, N R Cottrell, 1984

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8. Determination of Ochratoxin A in Grain by Monoclonal Antibody-Based Enzyme-Linked Immunosorbent Assay. [R] . Yang, C., Luo, X., Liu, C., 1994

机译：单克隆抗体酶联免疫吸附法测定谷物中赭曲霉毒素a的含量。

Enzyme-linked immunosorbent assays based on polyclonal and monoclonal antibodies for rotavirus detection.

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