...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Clinical Microbiology >Rapid detection of respiratory syncytial virus in nasopharyngeal aspirates by a commercial enzyme immunoassay.
【24h】

Rapid detection of respiratory syncytial virus in nasopharyngeal aspirates by a commercial enzyme immunoassay.

机译：通过商业酶免疫法快速检测鼻咽抽吸物中的呼吸道合胞病毒。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A commercial enzyme immunoassay (EIA) for the rapid detection of respiratory syncytial virus (RSV) in respiratory secretions was evaluated by comparison with both virus isolation in HEp-2 cells and indirect immunofluorescence (IFA) staining of exfoliated respiratory cells. Initial examination of 80 nasopharyngeal aspirates collected from infants with acute respiratory illness showed that the RSV EIA was positive for 21 of 24 specimens positive by virus isolation or IFA (87.5% sensitivity) and negative for 53 of 56 specimens negative by virus isolation and IFA (95% specificity). The EIA appears to be an acceptable and more rapid test than virus isolation for the detection of RSV, especially for laboratories in which prompt inoculation of specimens is not always possible. IFA staining with commercial bovine anti-RSV serum was found to be the most sensitive and rapid test for the detection of RSV. However, three of four specimens positive by IFA and negative by virus isolation were not cultured under optimal conditions. In addition, the IFA test requires a highly trained technologist to interpret the staining results.
机译：通过比较HEp-2细胞中的病毒分离和脱落的呼吸细胞的间接免疫荧光（IFA）染色，评估了用于快速检测呼吸道分泌物中的呼吸道合胞病毒（RSV）的商业酶免疫分析（EIA）。初步检查了80例急性呼吸系统疾病婴儿的鼻咽抽吸物，结果表明，通过病毒分离或IFA阳性的24个标本中有21个的RSV EIA阳性（灵敏度为87.5％），通过病毒分离和IFA阴性的56个标本中的53个中的RSV EIA阴性（ 95％的特异性）。对于检测RSV而言，EIA似乎是一种比病毒分离更可接受且更快速的测试方法，尤其是对于并非总是能够迅速接种标本的实验室而言。商业牛抗RSV血清的IFA染色被发现是检测RSV的最灵敏，最快速的测试。但是，在最佳条件下，未培养出IFA阳性和病毒分离阴性的四个标本中的三个。此外，IFA测试需要训练有素的技术人员来解释染色结果。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号