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首页> 外文期刊>Journal of Clinical Microbiology >Characterization of enteroinvasive Escherichia coli strains isolated from children with diarrhea in Chile.
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Characterization of enteroinvasive Escherichia coli strains isolated from children with diarrhea in Chile.

机译：在智利从腹泻儿童中分离出的肠侵袭性大肠杆菌菌株的特征。

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Analysis of stool samples from 912 cases of diarrhea among Chilean infants and 1,112 controls resulted in the isolation of 17 enteroinvasive Escherichia coli (EIEC) strains from diarrhea cases (1.9%) and 3 EIEC from the asymptomatic controls (0.3%). Biochemical analysis of the 20 isolates showed variability among them. However, the majority were lysine decarboxylase negative and nonmotile and utilized sodium acetate. The strains belonged to the O groups 28ac, 124, 143, or 144 or were untypable with the antisera used. Most of them had conjugative plasmids which mediated multiple antibiotic resistance. There was a strong correlation in this group of strains between a positive Sereny test, the presence of a plasmid of 120 megadaltons, and hybridization with the invasiveness probe, an HindIII fragment derived from the plasmid pPS15A. The isolates had a wide range of plasmid profiles. Bioassays and colony and Southern hybridization tests with iron uptake DNA probes indicated that 80% of the EIEC strains produced aerobactin and expressed its receptor, the genes for which are known to be chromosomally located.
机译：对智利婴儿912例腹泻病例和1,112例对照的粪便样本进行分析，结果从腹泻病例中分离出17例肠侵害性大肠杆菌（EIEC）菌株（1.9％），从无症状对照中分离出3例EIEC（0.3％）。对20个分离株的生化分析表明它们之间存在差异。但是，大多数是赖氨酸脱羧酶阴性且不能运动的，并且使用了乙酸钠。这些菌株属于O组28ac，124、143或144，或者不能与所使用的抗血清结合。它们中的大多数具有介导多种抗生素抗性的结合质粒。这组菌株之间在Sereny阳性试验，120兆道尔顿质粒的存在以及与侵袭性探针（源自质粒pPS15A的HindIII片段）的杂交之间存在很强的相关性。分离物具有广泛的质粒谱。用铁摄取DNA探针进行的生物测定，菌落和Southern杂交试验表明，80％的EIEC菌株产生了气杆菌素并表达了其受体，已知该基因的基因位于染色体上。

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