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首页> 外文期刊>Journal of Clinical Microbiology >Identification of EF group 22 campylobacters from gastroenteritis cases as Campylobacter concisus.
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Identification of EF group 22 campylobacters from gastroenteritis cases as Campylobacter concisus.

机译：从肠胃炎病例中确认EF组22弯曲杆菌为结膜弯曲杆菌。

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摘要

EF (E. Falsen) group 22, a group of Campylobacter strains sorted out by routine immunotyping among unidentified or misidentified human nonoral clinical specimens, was characterized by numerical analysis of gel electrophoretic protein profiles and immunotyping. The protein electrophoretic and immunotyping analyses, DNA:DNA hybridizations, and the DNA base composition demonstrated unambiguously that all EF group 22 strains belong to Campylobacter concisus. EF group 22 strains have DNA binding values of at least 42% with the type strain of C. concisus, showing a considerable degree of genomic heterogeneity. The isolation from blood, esophagus, stomach, duodenum, and feces of humans in association with different gastrointestinal disorders considerably extends the clinical significance of this species. Our results indicate that sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunotyping are excellent tools for the identification of the fastidious C. concisus strains and relatives.
机译：EF（E. Falsen）22组是通过常规免疫分型在未鉴定或未鉴定的人类非口腔临床标本中筛选出的弯曲杆菌菌株，其特征在于通过凝胶电泳蛋白质谱和免疫分型的数值分析来表征。蛋白质电泳和免疫分型分析，DNA：DNA杂交和DNA碱基组成明确表明，所有EF组22菌株均属于结膜弯曲杆菌。 EF组22菌株与圆锥形隐孢子虫菌株的DNA结合值至少为42％，显示出相当程度的基因组异质性。从人类的血液，食道，胃，十二指肠和粪便中分离出来，并伴有不同的胃肠道疾病，大大扩展了该物种的临床意义。我们的结果表明，十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和免疫分型是鉴别伪劣C. conconsus菌株及其亲属的极佳工具。

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