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首页> 外文期刊>Journal of Clinical Microbiology >Phage typing of Staphylococcus intermedius.
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Phage typing of Staphylococcus intermedius.

机译：中间葡萄球菌的噬菌体分型。

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Staphylococcus intermedius, a coagulase-positive staphylococcal species, is a common canine pathogen and a rare human wound pathogen. A total of 145 strains of S. intermedius (ATCC 29663, 4 reference strains, 4 human isolates, 44 canine infection isolates, and 92 isolates from canine gingiva) were screened for lysogenic phage by a modified Fisk method. Nineteen phage preparations were prepared for preliminary typing experiments. Lytic activity was observed on 93 of 145 (64.1%) isolates, yielding 44 lytic patterns with individual strains susceptible to one or more phages. Five phages lysed only a single strain, but lytic patterns varied from 1 to 11 lytic phages per isolate. A distinct lytic pattern did not separate canine or human wound isolates from canine gingival isolates. All human wound isolates fell into the two most common canine gingival or wound patterns; the single human nasopharyngeal isolate was not lysed by any phage. Twenty-two of 44 (55%) canine wound isolates and 65 of 92 (71%) gingival isolates yielded lytic patterns. Lysogenic phages are common in S. intermedius. This preliminary study suggests that phage typing may be a useful tool in distinguishing epidemiologically related strains.
机译：中间葡萄球菌是一种凝固酶阳性葡萄球菌，是常见的犬病原体和罕见的人类伤口病原体。通过改良的Fisk方法筛选了共145株中间链球菌（ATCC 29663、4株参考菌株，4株人分离株，44株犬感染株和92株来自牙龈的菌株）的溶原性噬菌体。制备了十九种用于初步分型实验的噬菌体制剂。在145个分离物中的93个（64.1％）分离物中观察到了裂解活性，产生了44种裂解模式，其中单个菌株易感一种或多种噬菌体。五个噬菌体仅裂解一个菌株，但每个分离株的裂解模式为1到11个裂解噬菌体。独特的裂解模式没有将犬或人伤口分离物与犬齿龈分离物分开。所有人类伤口分离物均分为两种最常见的犬齿龈或伤口模式;单个人鼻咽分离物未被任何噬菌体裂解。 44个犬齿伤口分离物中的22个（55％）和92个牙龈分离物中的65个（71％）产生了溶解模式。溶原性噬菌体在中间链球菌中很常见。这项初步研究表明，噬菌体分型可能是区分流行病学相关菌株的有用工具。

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