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首页> 外文期刊>Journal of Clinical Microbiology >Latex agglutination-negative methicillin-resistant Staphylococcus aureus recovered from neonates: epidemiologic features and comparison of typing methods.
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Latex agglutination-negative methicillin-resistant Staphylococcus aureus recovered from neonates: epidemiologic features and comparison of typing methods.

机译：新生儿乳胶凝集阴性耐甲氧西林金黄色葡萄球菌的流行病学特征和分型方法的比较。

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An unusual strain of methicillin-resistant Staphylococcus aureus (MRSA) was repeatedly isolated from infants in a newborn special care unit (NBSC) and a newborn intensive care unit. Between January 1989 and March 1990, approximately 100 isolates from infected or colonized infants were recovered. Surveillance cultures taken during this time revealed a 20% colonization rate, which was defined as recovery of MRSA from the nares, umbilicus, or groin. Isolates were identified as S. aureus by tube coagulase reactivity and heat-stable nuclease production but were unreactive in a latex agglutination assay. Representative isolates that were collected during the outbreak and that were found to share the latex agglutination assay-negative phenotype were compared by antibiogram (12 isolates), bacteriophage typing (20 isolates), capsular polysaccharide typing (30 isolates), and plasmid as well as chromosomal DNA analyses (20 isolates). All isolates known to be associated with the outbreak had nearly identical antibiograms and were notably susceptible to clindamycin. Staphylococcal bacteriophage typing was not useful in determining the relatedness of the isolates, since the majority were nontypeable. Plasmid pattern analysis revealed one large plasmid (approximately 100 kb) of equivalent size among the isolates. Capsular polysaccharide typing revealed that 14 of 30 isolates tested were type 5. Isolates identified in children at two other hospitals in the city which were also unreactive by the latex agglutination assay and clindamycin susceptible had plasmid and antibiogram patterns identical to those of isolates from the NBSC. Pulsed-field gel electrophoresis of restriction enzyme-digested genomic DNAs from the outbreak isolates demonstrated identical patterns which could be clearly differentiated from those of other unrelated MRSA. The strain from the NBSC is, therefore, unique and underscores the need for caution in interpreting the latex agglutination reactivities of MRSA isolates.
机译：在新生儿特殊监护室（NBSC）和新生儿重症监护室中，反复从婴儿中分离出一种耐甲氧西林金黄色葡萄球菌（MRSA）的异常株。在1989年1月至1990年3月之间，从感染或定植的婴儿中回收了大约100株分离株。在这段时间内进行的监视培养显示出20％的定殖率，这被定义为从鼻孔，脐部或腹股沟中回收MRSA。通过管凝结酶反应性和热稳定的核酸酶产生将分离物鉴定为金黄色葡萄球菌，但在乳胶凝集测定中则不反应。通过抗菌素谱图（12株），噬菌体分型（20株），荚膜多糖分型（30株），质粒以及染色体DNA分析（20个分离株）。已知与该暴发有关的所有分离株的抗菌素谱图几乎相同，并且对克林霉素特别敏感。葡萄球菌噬菌体分型在确定分离株的相关性方面没有用，因为大多数是不可分型的。质粒模式分析揭示了分离物中一个大小相等的大质粒（约100 kb）。荚膜多糖分型显示，在30个分离株中有14个是5型。在该市另外两家医院的儿童中鉴定出的分离物，这些分离物也未通过乳胶凝集测定反应，并且对克林霉素敏感，其质粒和抗菌谱特征与NBSC分离物相同。爆发分离物中限制性内切酶消化的基因组DNA的脉冲场凝胶电泳显示出相同的模式，可以与其他不相关的MRSA明显区别开来。因此，来自NBSC的菌株是独特的，强调了在解释MRSA分离物的乳胶凝集反应性时需要谨慎。

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