...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Clinical Microbiology >Confirmation of human cytomegalovirus by reverse passive hemagglutination with monoclonal antibodies reactive to the major glycosylated peptide (GP-66).
【24h】

Confirmation of human cytomegalovirus by reverse passive hemagglutination with monoclonal antibodies reactive to the major glycosylated peptide (GP-66).

机译：通过对主要糖基化肽（GP-66）有反应性的单克隆抗体的反向被动血凝法确认人巨细胞病毒。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Sheep erythrocytes coated with three monoclonal antibodies, each reactive to a different epitope of the 66-kilodalton cytomegalovirus (CMV) matrix protein, were used in a reverse passive hemagglutination test with CMV-infected cell lysate to identify and confirm the CMV. The test is specific only for CMV, since 5 laboratory strains of CMV (AD169, Davis, Espilat, C-87, and Towne) and 10 clinical isolates reacted well, but uninfected MRC-5 cell lysate, lysates of herpes simplex virus types 1 and 2, varicella-zoster virus, and adenoviruses did not react. The reactive CMV lysate was confirmed by the pretreatment of CMV lysate with the three monoclonal antibodies followed by the addition of antibody-coated erythrocytes. The reverse passive hemagglutination test and the confirmatory blocking test are performed at the same time, requiring 2 h to complete. Since V-bottom microtiter 96-well plates and a 25-microliter pipette can be used to perform the test, it is ideal for CMV confirmation, especially when the equipment to read the fluorescent-antibody test or enzyme-linked immunosorbent assay is not available.
机译：涂有三种单克隆抗体的绵羊红细胞分别与66-千达尔顿巨细胞病毒（CMV）基质蛋白的不同表位有反应，用于CMV感染细胞裂解液的反向被动血凝试验，以鉴定和确认CMV。该测试仅针对CMV，因为5种CMV实验室菌株（AD169，Davis，Espilat，C-87和Towne）和10种临床分离株反应良好，但未感染的MRC-5细胞裂解物，单纯疱疹病毒1型裂解物2，水痘带状疱疹病毒和腺病毒没有反应。通过用三种单克隆抗体对CMV裂解物进行预处理，然后添加抗体包被的红细胞，证实了反应性CMV裂解物。反向被动血凝试验和确认性阻断试验同时进行，需要2小时才能完成。由于可以使用V型底部微量滴定板96孔板和25微升移液管进行检测，因此非常适合CMV确认，尤其是在没有读取荧光抗体检测或酶联免疫吸附测定的设备时。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号