...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Clinical Microbiology >Application of Competitive PCR to Cerebrospinal Fluid Samples from Patients with Herpes Simplex Encephalitis
【24h】

Application of Competitive PCR to Cerebrospinal Fluid Samples from Patients with Herpes Simplex Encephalitis

机译：竞争PCR在单纯疱疹性脑炎患者脑脊液样本中的应用

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The purpose of the present study was to determine if the quantity of herpes simplex virus (HSV) DNA in the cerebrospinal fluid (CSF) of patients with herpes encephalitis would be useful in establishing the prognosis of the disease and to determine the effect of antiviral therapy on the clearance of viral DNA from the CSF. Quantitation of HSV DNA was done by constructing an internal standard (IS) from the glycoprotein B amplicon which had a 25-bp deletion between primer annealing sites. Each CSF specimen was coamplified with the IS and the ratio of the amount of HSV/amount of IS was compared to the ratios on a standard curve constructed with the same IS plus known amounts of HSV DNA. CSF specimens were available from 16 patients who were treated with intravenous acyclovir, and the amount of HSV DNA ranged from <25 to 18,000 copies per μl in CSF obtained before or within 4 days of the initiation of acyclovir therapy. Patients with >100 copies of HSV DNA per μl were older, were found by computed tomography to have lesions, and had poorer outcomes than patients with <100 copies. Follow-up CSF specimens were available from seven patients. In six of these seven patients, the HSV DNA levels decreased during therapy. One patient had a twofold increase in HSV DNA levels after 1 week of therapy and died on day 8. The application of this assay may be helpful in establishing the prognosis and in the monitoring of patients with herpes simplex encephalitis.
机译：本研究的目的是确定疱疹性脑炎患者脑脊液（CSF）中单纯疱疹病毒（HSV）DNA的量是否可用于确定疾病的预后并确定抗病毒治疗的效果从脑脊液清除病毒DNA。通过从糖蛋白B扩增子构建内标（IS）进行HSV DNA定量，该内标在引物退火位点之间缺失25 bp。将每个CSF标本与IS进行扩增，并将HSV量/ IS量的比率与由相同IS和已知量的HSV DNA构成的标准曲线上的比率进行比较。可以从16名接受阿昔洛韦静脉注射治疗的患者中获取CSF标本，在开始使用阿昔洛韦治疗之前或4天之内，CSF中HSV DNA的量范围为每μl<25至18,000拷贝。 HSV DNA> 100拷贝/μl的患者年龄较大，通过计算机断层扫描发现其有病灶，且结果<100拷贝的患者较差。可以从7位患者中获得随访的CSF标本。在这七名患者​​中的六名中，治疗期间HSV DNA水平下降。一名患者在治疗1周后HSV DNA水平增加了两倍，并在第8天死亡。该测定方法的应用可能有助于确定单纯疱疹性脑炎患者的预后和监测。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号