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首页> 外文期刊>Journal of Clinical Microbiology >Phenotypic characterization, cellular fatty acid composition, and DNA relatedness of aerococci and comparison to related genera.
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Phenotypic characterization, cellular fatty acid composition, and DNA relatedness of aerococci and comparison to related genera.

机译：表型特征，细胞脂肪酸组成，和球菌的DNA相关性以及与相关属的比较。

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摘要

Aerococci can be misidentified as streptococci, enterococci, pediococci, lactococci, or leuconostocs. To distinguish the genus and determine if another species is needed in the present taxon, we analyzed 37 aerococci for cellular fatty acids and compared them with 377 strains of gram-positive cocci, including the species type strains from each of the related genera. The cellular fatty acid profile of aerococci was distinguishable from other genera. Two relatively novel fatty acids found in the aerococci were identified as C16:1 omega 9c and C16:1 omega 9t. Eleven strains of aerococci (including a strain originally identified as "Gaffkya" species) were chosen for DNA-DNA reassociation studies with the type strain Aerococcus viridans ATCC 11563; DNAs from eight of these strains were more than 75% related to the type strain and had 1 to 4% divergence in related sequences. The remaining three strains were 60 to 70% related to the type strain, had 7 to 11.5% divergence, and may represent a second species, Aerococcus genospecies 2. beta-Glucuronidase, alpha-galactosidase, and beta-galactosidase were useful in characterizing the aerococci.
机译：Aerococci可能被误认为是链球菌，肠球菌，pediococci，lactoococci或leuconostocs。为了区分属并确定本分类单元中是否需要其他物种，我们分析了37种细球菌的细胞脂肪酸并将它们与377株革兰氏阳性球菌进行了比较，包括每个相关属的物种类型。 Aerococci的细胞脂肪酸谱可与其他属区分开。在航空球菌中发现的两种相对较新的脂肪酸被鉴定为C16：1ω9c和C16：1ω9t。选择了11种拟球菌菌株（包括最初鉴定为“ Gaffkya”菌种的菌株）与Aerococcus viridans ATCC 11563型菌株进行DNA-DNA重结合研究。来自这些菌株中的八个的DNA与该类型菌株相关的超过75％，并且在相关序列中具有1-4％的差异。其余三个菌株与该类型菌株有60％到70％的相关性，有7至11.5％的差异，可能代表第二个物种，Aerococcus genospecies 2。航空球菌。

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