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首页> 外文期刊>Journal of Clinical Microbiology >DNA large restriction fragment patterns of sporadic and epidemic nosocomial strains of Mycobacterium chelonae and Mycobacterium abscessus.
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DNA large restriction fragment patterns of sporadic and epidemic nosocomial strains of Mycobacterium chelonae and Mycobacterium abscessus.

机译：散发和流行的分枝杆菌和脓肿分枝杆菌的医院菌株的DNA大限制性片段模式。

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Large restriction fragment (LRF) pattern analysis of genomic DNA using pulsed-field gel electrophoresis was performed on three reference strains, 32 sporadic isolates, and 92 nosocomial isolates from 12 epidemics of Mycobacterium chelonae and Mycobacterium abscessus. Only 17 of 30 (57%) unrelated strains of M. abscessus, compared with 10 of 11 (91%) of M. chelonae strains, gave satisfactory DNA extractions, with the remainder resulting in highly fragmented DNA. DraI, AsnI, XbaI, and SpeI gave satisfactory LRF patterns. Sporadic isolates of the two species had highly variable LRF patterns, except for one reference strain and one sporadic isolate of M. chelonae that differed by only two to five bands. Evaluation of repeat isolates from five patients monitored for 8 months to 13 years (mean, 5.8 years) revealed LRF patterns to be stable, with changes of not more than two bands. LRF analysis of the seven nosocomial outbreaks with evaluable DNA revealed identical patterns in most or all of the patient isolates and in three outbreaks revealed identity with environmental isolates. These outbreaks included endoscope contamination, postinjection abscesses, and surgical wound infections. LRF analysis of genomic DNA is a useful technique for epidemiologic studies of M. abscessus and M. chelonae, although improved technology is needed for the approximately 50% of strains of M. abscessus with unsatisfactory DNA extractions.
机译：使用脉冲场凝胶电泳对三个参考菌株，32个散发性分离株和来自12个流行分枝杆菌和脓肿分枝杆菌的92个医院分离株进行了基因组DNA的大限制性片段（LRF）模式分析。 30个无关的脓肿分支杆菌菌株中只有17个（57％），而chelonae分离的11个菌株中只有10个菌株（91％）具有令人满意的DNA提取，其余的则导致高度片段化的DNA。 DraI，AsnI，XbaI和SpeI给出了令人满意的LRF模式。这两个物种的零星分离株具有高度可变的LRF模式，除了一个参考菌株和一个零食的chelonae零星分离株之间仅相差2至5个带。对从监测到的8个月至13年（平均5.8年）的五名患者的重复分离株进行的评估显示，LRF模式稳定，变化范围不超过两个谱带。对具有可评估DNA的7个医院暴发的LRF分析显示，在大多数或所有患者分离株中，模式相同，而在三个暴发中则与环境分离株相同。这些暴发包括内窥镜污染，注射后脓肿和外科伤口感染。基因组DNA的LRF分析是一种用于脓肿支原体和chelonae流行病学研究的有用技术，尽管大约50％的脓肿支原体菌株需要改进的技术，但DNA提取效果不理想。

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