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首页> 外文期刊>Journal of Clinical Microbiology >Evaluation of counterimmunoelectrophoresis for serotyping Actinobacillus pleuropneumoniae isolates and detection of type-specific antigens in lungs of infected pigs.
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Evaluation of counterimmunoelectrophoresis for serotyping Actinobacillus pleuropneumoniae isolates and detection of type-specific antigens in lungs of infected pigs.

机译：评价抗免疫电泳法对胸膜肺炎放线杆菌血清分型的方法以及在感染猪肺中检测类型特异性抗原。

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A rapid, simple, and accurate counterimmunoelectrophoresis technique was developed for serotyping cultures of Actinobacillus pleuropneumoniae as well as for detection of their type-specific antigens in the lung tissues of infected pigs. The counterimmunoelectrophoresis test correctly identified all of the reference antigens and more than 99% of 1,200 field isolates of A. pleuropneumoniae representing the 12 established serotypes within 1 h. Counterimmunoelectrophoresis and coagglutination tests did not differ broadly in sensitivity from each other. Both procedures were more rapid and more sensitive than immunodiffusion and indirect hemagglutination tests. A total of 355 lung tissue samples (130 lungs of pigs that died because of acute respiratory problems, 125 lungs of pigs from herds with chronically infected pleuropneumonia, and 100 lungs from apparently healthy pigs at the slaughterhouse) were examined for the presence of A. pleuropneumoniae type-specific antigens by counterimmunoelectrophoresis, coagglutination, and immunodiffusion tests. A. pleuropneumoniae type-specific antigen was found in all 55 samples from which the bacteria had earlier been isolated and in 27 specimens in which they had not been found. Detection of antigen in the lung tissues by coagglutination and counterimmunoelectrophoresis tests was found to be much simpler and much more rapid than conventional culture isolation. Both counterimmunoelectrophoresis and coagglutination tests were found extremely useful in the diagnosis of acute cases of porcine pleuropneumonia. However, these techniques were able to detect only some of the chronically infected carrier pigs.
机译：开发了一种快速，简单和准确的对免疫电泳技术，用于胸膜肺炎放线杆菌的血清分型培养以及在感染猪的肺组织中检测其类型特异性抗原。抗免疫电泳测试正确地鉴定了所有参考抗原和代表1小时内代表12种血清型的胸膜肺炎链球菌的1,200种田间分离株中的99％以上。对抗免疫电泳和凝集试验的灵敏度没有太大差异。与免疫扩散和间接血凝试验相比，这两种方法都更快，更灵敏。检查了总共355个肺组织样本（由于急性呼吸系统疾病而死亡的130头猪肺，来自慢性感染性胸膜肺炎的猪群的125肺和屠宰场中看似健康的猪的100肺）是否存在A.胸膜肺炎类型特异性抗原通过反免疫电泳，凝集和免疫扩散测试。在早先从中分离出细菌的所有55个样本中和未发现细菌的27个样本中发现了胸膜肺炎链球菌类型特异性抗原。通过凝集和反免疫电泳测试发现肺组织中的抗原比传统的培养分离方法更简单，更快速。发现反免疫电泳和凝集试验在诊断猪胸膜肺炎的急性病例中非常有用。但是，这些技术只能检测到一些慢性感染的携带猪。

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