Identification of spheroplast-like agents isolated from tissues of patients with Crohn's disease and control tissues by polymerase chain reaction.

S Wall; Z M Kunze; S Saboor; I Soufleri; P Seechurn; R Chiodini; J J McFadden

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Identification of spheroplast-like agents isolated from tissues of patients with Crohn's disease and control tissues by polymerase chain reaction.

机译：通过聚合酶链反应鉴定从克罗恩病患者组织和对照组织中分离的原生质球样药物。

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Mycobacterium paratuberculosis has been isolated from tissue taken from patients with Crohn's disease and has been implicated in the etiology of this disease. On culture, the organisms appear initially as cell wall-deficient, spheroplast-like forms that are difficult to identify by conventional techniques. Here we examine 30 unidentified cultures by the polymerase chain reaction using primers specific for M. paratuberculosis, Mycobacterium tuberculosis, and Mycobacterium avium restriction fragment length polymorphism type A/I and also by a non-species-specific mycobacterial polymerase chain reaction. Six of these cultures, all from Crohn's disease, were shown to contain DNA from M. paratuberculosis. Cultures from both Crohn's disease and controls were found to contain mycobacterial DNA of unknown specific origin.

机译：已经从取自克罗恩氏病患者的组织中分离了副结核分枝杆菌，并与该病的病因有关。在培养时，这些生物最初表现为细胞壁缺陷，原生质球状形式，难以通过常规技术鉴定。在这里，我们通过聚合酶链反应，使用对副结核分枝杆菌，结核分枝杆菌和鸟分枝杆菌限制性片段长度多态性类型A / I特异性的引物，以及非物种特异性分枝杆菌聚合酶链反应，检查了30种未鉴定的培养物。这些培养物中的六种均来自克罗恩氏病，显示含有副结核分枝杆菌的DNA。发现来自克罗恩氏病和对照的培养物中均含有未知来源的分枝杆菌DNA。

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《Journal of Clinical Microbiology》 |1993年第5期|共5页
作者
S Wall; Z M Kunze; S Saboor; I Soufleri; P Seechurn; R Chiodini; J J McFadden;
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中图分类医学微生物学（病原细菌学、病原微生物学）;
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机译：膜和组织的二次离子质谱和光学成像分析：A部分：用于控制蛋白质药物递送的可生物降解聚合物膜，B部分：异种移植组织中的化学治疗剂。
6. Identification of spheroplast-like agents isolated from tissues of patients with Crohns disease and control tissues by polymerase chain reaction. [O] . S Wall, Z M Kunze, S Saboor, 1993

机译：通过聚合酶链反应鉴定从克罗恩氏病患者组织和对照组织中分离的原生质球样药物。
7. Identification of spheroplast-like agents isolated from tissues of patients with Crohn's disease and control tissues by polymerase chain reaction [O] . S Wall, Z M Kunze, S Saboor, 1993

机译：通过聚合酶链反应鉴定克罗恩疾病患者组织和对照组织患者组织的鉴定

Identification of spheroplast-like agents isolated from tissues of patients with Crohn's disease and control tissues by polymerase chain reaction.

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