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首页> 外文期刊>Journal of Clinical Microbiology >Characterization of Neisseria elongata subsp. glycolytica isolates obtained from human wound specimens and blood cultures.
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Characterization of Neisseria elongata subsp. glycolytica isolates obtained from human wound specimens and blood cultures.

机译:延性奈瑟氏球菌亚种的鉴定。从人伤口标本和血液培养物中获得的糖酵解分离物。

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Four slightly yellow-pigmented, alpha-hemolytic, gram-negative coccobacilli, three from wound specimens and one from multiple blood cultures of a patient with endocarditis, were identified as Neisseria elongata subsp. glycolytica on the basis of their overall biochemical and genetic similarities to this subspecies. These strains resembled N. elongata in their guanine-plus-cytosine contents (55.6 to 57.1 mol%) and in their overall cellular fatty acid profiles, which are characterized by large amounts of 16:0, 16:1 omega 7c, and 18:1 omega 7c fatty acids. Their identities were confirmed by species-level DNA relatedness (hydroxyapatite method) to the type strains of all three N. elongata subspecies. The biochemical profiles and cultural characteristics of these strains resembled those of the type strain of N. elongata subsp. glycolytica except for the production of a weak yellow growth pigment and alpha-hemolysis on sheep blood agar. They differed from N elongata subsp. elongata by the production of catalase, by the production of alpha-hemolysis on sheep blood agar, and by acid production from D-glucose. They differed from N. elongata subsp. nitroreducens by the production of catalase and an inability to reduce nitrate. These studies suggest a pathogenic potential for N. elongata subsp. glycolytica, usually considered to be a transient colonizer in humans.
机译:心内膜炎患者的四个淡黄色色素,α-溶血性,革兰氏阴性球菌,三个来自伤口标本,一个来自多种血液培养物的细菌被鉴定为延性奈瑟菌。糖酵解糖的总体生化和遗传相似性。这些菌株的鸟嘌呤加胞嘧啶含量(55.6至57.1 mol%)和总体细胞脂肪酸谱类似于长株猪笼草,其特征是大量的16:0、16:1Ω7c和18: 1欧米茄7c脂肪酸。它们的身份已通过与所有三个长夜蛾亚种的类型菌株的物种水平DNA相关性(羟基磷灰石法)得到证实。这些菌株的生化特性和培养特性与长型猪笼草亚种的菌株相似。除了在羊血琼脂上产生微弱的黄色生长色素和α-溶血作用以外,还需要糖酵解。它们不同于N elongata亚种。通过产生过氧化氢酶,通过在羊血琼脂上产生α-溶血酶和通过从D-葡萄糖产生酸来延长植物。它们不同于猪笼草亚种。过氧化氢酶的产生导致硝酸还原酶还原,并且无法还原硝酸盐。这些研究表明长鼻猪笼草亚种具有潜在的致病性。溶果糖,通常被认为是人类的短暂定居者。

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