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首页> 外文期刊>Journal of Clinical Microbiology >Auxotrophy of Burkholderia (Pseudomonas) cepacia from cystic fibrosis patients.
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Auxotrophy of Burkholderia (Pseudomonas) cepacia from cystic fibrosis patients.

机译：囊性纤维化患者的伯克霍尔德氏菌（假单胞菌）洋葱的营养不良。

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The nutritional status of 89 isolates of Burkholderia cepacia from 81 cystic fibrosis (CF) patients was evaluated. Forty of the isolates, from 38 patients, were not able to grow in a minimal medium containing glucose and mineral salts only and were thus auxotrophs. In contrast, all of 29 isolates from non-CF (clinical and environmental) sources were prototrophic. Addition of a pool of amino acids to the minimal medium was sufficient to promote growth of all tested CF auxotrophic isolates. Indeed, phenylalanine, tyrosine, cysteine, methionine, and histidine alone or in combination were required for growth by the majority of the nutritionally deficient B. cepacia isolates. Furthermore, extracts of sputum from CF patients, when added to minimal medium, promoted growth of 29 auxotrophic B. cepacia isolates regardless of their amino acid requirements. Finally, auxotrophic and prototrophic isolates from the same patient exhibited a conserved genotype, as determined by macrorestriction analysis of chromosomal DNA. These results suggest that the auxotrophic mutants are selected from the prototrophic population and maintained by the nutritionally rich environment of the CF airways.
机译：评价了来自81名囊性纤维化（CF）患者的89种分离的洋葱伯克霍尔德菌的营养状况。来自38个患者的40个分离株不能在仅包含葡萄糖和矿物质盐的基本培养基中生长，因此是营养缺陷型。相比之下，来自非CF（临床和环境）来源的29种分离株均为营养缺陷型。向基本培养基中添加氨基酸池足以促进所有测试的CF营养缺陷型分离物的生长。实际上，大多数营养缺乏的洋葱伯克霍尔德菌分离株单独或组合需要苯丙氨酸，酪氨酸，半胱氨酸，蛋氨酸和组氨酸来生长。此外，将CF患者的痰液提取物添加至基本培养基后，无论其氨基酸需求如何，均可促进29种营养缺陷型洋葱伯克霍尔德菌的生长。最后，来自同一患者的营养缺陷型和原养型分离株表现出保守的基因型，这是通过染色体DNA的宏观限制性分析确定的。这些结果表明营养缺陷型突变体选自营养缺陷型种群，并由CF气道的营养丰富的环境维持。

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