...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Clinical Microbiology >Rapid serological technique for typing herpes simplex viruses.
【24h】

Rapid serological technique for typing herpes simplex viruses.

机译：快速血清学技术，用于输入单纯疱疹病毒。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A rapid technique is described which can accurately identify a herpes simplex virus (HSV) isolate as type 1 or type 2. Filter paper disks were used to immobilize viral antigens, which were then identified by means of an (125)I-labeled staphylococcal protein A immunoassay. The assay was performed in a specially designed 96-well filtration device which served as both an incubation chamber and a filter manifold. By using this system and cross-absorbed antisera to HSV types 1 and 2, 69 coded clinical isolates of HSV were correctly and unequivocally typed. HSV was also clearly distinguished from varicella-zoster virus and cytomegalovirus. This assay can be rapidly executed (less than 2 h) and yielded an objective endpoint; it required only minute quantities of typing sera and can be easily performed with the cells from a single infected roller tube culture. Thus, it can be used to type initial clinical isolates of HSV, yielding results within hours after the first appearance of cytopathic effects in the culture used for primary virus isolation. Moreover, it is particularly well suited to the simultaneous analysis of many specimens and is amenable to automation. These characteristics suggest that this (125)I-labeled staphylococcal protein A immunofiltration technique will be applicable to the rapid identification of other herpesviruses, as well as other clinical isolates.
机译：描述了一种快速技术，该技术可以准确地将单纯疱疹病毒（HSV）分离株鉴定为1型或2型。滤纸盘用于固定病毒抗原，然后通过（125）I标记的葡萄球菌蛋白进行鉴定免疫测定。该测定是在专门设计的96孔过滤设备中进行的，该设备既用作孵育室，又用作过滤器歧管。通过使用该系统以及针对HSV 1型和2型的交叉吸收抗血清，正确，明确地分了69种编码的HSV临床分离株。 HSV也明显区别于水痘带状疱疹病毒和巨细胞病毒。该测定可快速执行（少于2小时）并产生客观终点。它仅需极少量的打字血清，并且可以很容易地用单个感染的滚子管培养物中的细胞进行。因此，它可用于对HSV的初始临床分离株进行分型，在用于原代病毒分离的培养物中首次出现细胞病变效应后数小时内即可得到结果。此外，它特别适合于同时分析许多样本，并且适合自动化。这些特征表明，这种（125）I标记的葡萄球菌蛋白A免疫过滤技术将适用于其他疱疹病毒以及其他临床分离株的快速鉴定。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号