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首页> 外文期刊>Journal of Clinical Microbiology >Kinetic-dependent enzyme-linked immunosorbent assay for detection of antibodies to Legionella pneumophila.
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Kinetic-dependent enzyme-linked immunosorbent assay for detection of antibodies to Legionella pneumophila.

机译：动力学依赖的酶联免疫吸附测定法，用于检测抗军团杆菌的抗体。

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A semiautomated, kinetic-dependent, enzyme-linked immunosorbent assay (K-ELISA) was adapted to detect serum antibodies to Legionella pneumophila. In a comparative study, 158 human serum samples (79 pairs) were tested by K-ELISA and the standard indirect immunofluorescence assay for determination of antibody levels to L. pneumophila serogroup 1. K-ELISA determinations were made by using a serogroup-specific antigen or a preparation (unfractionated antigen) which contained both common antigen and serogroup-specific reactivity. There was good correlation between the immunofluorescence assay and the K-ELISA by using either antigen, although greater correlation was achieved with the unfractionated antigen (coefficients of correlation, 0.894 with unfractionated antigen and 0.841 with serogroup-specific antigen). These results indicate that the K-ELISA is a reliable alternative to the immunofluorescence assay for serologically diagnosing legionellosis.
机译：半自动化，动力学依赖，酶联免疫吸附测定（K-ELISA）适用于检测抗军团杆菌的血清抗体。在一项比较研究中，通过K-ELISA和标准间接免疫荧光测定法测试了158个人血清样品（79对），以测定针对肺炎嗜血杆菌血清群1的抗体水平。通过使用血清群特异性抗原进行K-ELISA测定或含有普通抗原和血清群特异性反应性的制剂（未分级抗原）。尽管使用未分离的抗原获得了更大的相关性（相关系数，未分离的抗原为0.894，血清组特异性抗原为0.841），但是使用这两种抗原在免疫荧光测定和K-ELISA之间都具有良好的相关性。这些结果表明，K-ELISA是用于血清学诊断军团菌病的免疫荧光测定的可靠替代方法。

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