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首页> 外文期刊>Journal of Clinical Microbiology >Factors affecting Staphylococcus epidermidis growth in peritoneal dialysis solutions.
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Factors affecting Staphylococcus epidermidis growth in peritoneal dialysis solutions.

机译：腹膜透析液中影响表皮葡萄球菌生长的因素。

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Staphylococcus epidermidis is the most frequent cause of peritonitis complicating continuous ambulatory peritoneal dialysis. We studied factors that might influence the growth of S. epidermidis in commercially available peritoneal dialysis solution (PDS). Test strains were inoculated into PDS and incubated overnight at 37 degrees C. Samples were removed at appropriate intervals, bacterial counts were performed, and growth curves were constructed. We studied the effects of various osmolarities, the neutralization and acidification of fresh and spent PDS, and the effect of intraperitoneal dwell time on the ability PDS to support growth of S. epidermidis. In fresh PDS, numbers of bacteria remained constant after 24 h. No significant differences in growth were observed among PDS with 0.5, 1.5, 2.5, and 4.25% glucose. Neutralizing acidic fresh PDS had no effect on bacterial growth. However, growth did occur in spent PDS. PDS which was recovered after only 2 h in the peritoneal cavity supported growth to the same extent as did PDS recovered after 4 to 6 h. Mean log10 changes after 24 h of incubation were as follows: for fresh PDS, -1.3; after 2 h dwell time, 2.9; after 4 h dwell time, 1.9; and after 6 h dwell time, 1.3. Acidification of spent PDS to less than pH 6.35 produced less rapid growth; mean log10 increases after 24 h of incubation were 1.9 for pH 7.75, 1.6 for pH 6.35, 0.6 for pH 5.75, and 0.7 for pH 4.95. Fresh PDS of all available osmolarities neither supported the growth of S. epidermidis nor was bactericidal. Spent PDS supported bacterial growth, and this growth was partly independent of the neutralization which occurred during the dialysis.
机译：表皮葡萄球菌是腹膜炎并发非卧床腹膜透析的最常见原因。我们研究了可能影响市售腹膜透析液（PDS）中表皮葡萄球菌生长的因素。将测试菌株接种到PDS中，并在37℃下孵育过夜。以适当的间隔取出样品，进行细菌计数，并构建生长曲线。我们研究了各种渗透压的影响，新鲜和用过的PDS的中和和酸化以及腹膜内停留时间对PDS支持表皮葡萄球菌生长的能力的影响。在新鲜的PDS中，细菌数量在24小时后保持恒定。在葡萄糖浓度为0.5、1.5、2.5和4.25％的PDS中，未观察到生长的明显差异。中和酸性新鲜PDS对细菌生长没有影响。但是，用过的PDS确实发生了增长。仅在腹膜腔内2小时后恢复的PDS支持生长，其程度与4至6小时后恢复的PDS相同。孵育24小时后的平均log10变化如下：对于新鲜PDS，-1.3;在2小时的停留时间之后，为2.9;在4小时的停留时间之后，为1.9;在6小时的停留时间之后，为1.3。用过的PDS酸化至pH值低于6.35时，生长速度较慢;孵育24小时后的平均log10增加值对于pH 7.75为1.9，对于pH 6.35为1.6，对于pH 5.75为0.6，对于pH 4.95为0.7。所有渗透压的新鲜PDS都不支持表皮葡萄球菌的生长，也没有杀菌作用。用过的PDS支持细菌生长，并且这种生长部分独立于透析过程中发生的中和作用。

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