Dot immunoperoxidase assay using monoclonal antibody for direct detection of cytomegalovirus in urine samples.

M Zerbini; M Musiani; G Gentilomi; M La Placa

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Dot immunoperoxidase assay using monoclonal antibody for direct detection of cytomegalovirus in urine samples.

机译：使用单克隆抗体的点免疫过氧化物酶测定法可直接检测尿液样本中的巨细胞病毒。

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A rapid, simple dot immunoperoxidase assay for the direct detection of cytomegalovirus in clinical urine samples was developed. The assay was performed on nitrocellulose paper dotted with urine pellets free of cellular debris. Cytomegalovirus was detected with a monoclonal antibody to the capsid antigen, and the complex was visualized by immunoperoxidase staining. Positive reactions appeared as well-defined dark blue spots. Of the 87 urine samples examined, 10 proved positive in the dot immunoperoxidase assay, and 77 proved negative. The results agreed completely with the detection of cytomegalovirus-induced antigens in cell cultures inoculated with clinical specimens.

机译：开发了一种快速，简单的点免疫过氧化物酶测定法，用于直接检测临床尿液样本中的巨细胞病毒。该测定在硝化纤维素纸上进行，该纸上点缀着没有细胞碎片的尿丸。用抗衣壳抗原的单克隆抗体检测巨细胞病毒，并通过免疫过氧化物酶染色观察复合物。阳性反应表现为明确的深蓝色斑点。在所检查的87个尿液样本中，有10个在点免疫过氧化物酶测定法中呈阳性，77个呈阴性。该结果与用临床标本接种的细胞培养物中巨细胞病毒诱导的抗原的检测完全一致。

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《Journal of Clinical Microbiology》 |1987年第11期|共3页
作者
M Zerbini; M Musiani; G Gentilomi; M La Placa;
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中图分类医学微生物学（病原细菌学、病原微生物学）;
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专利

1. Rapid detection of cytomegalovirus in cell culture by indirect immunoperoxidase staining with monoclonal antibody to an early nuclear antigen. [J] . P D Swenson, M H Kaplan Journal of Clinical Microbiology . 1985,第5期

机译：通过对早期核抗原的单克隆抗体间接免疫过氧化物酶染色，可以快速检测细胞培养物中的巨细胞病毒。
2. Detection of specific immunoglobulin M antibodies to cytomegalovirus by using monoclonal antibody to immunoglobulin M in an indirect immunofluorescence assay. [J] . M Zerbini, M Musiani, G Gentilomi, Journal of Clinical Microbiology . 1986,第1期

机译：通过在间接免疫荧光测定中使用针对免疫球蛋白M的单克隆抗体，检测针对巨细胞病毒的特异性免疫球蛋白M抗体。
3. Dot immunoperoxidase assay for detection of parvovirus B19 antigens in serum samples. [J] . G Gentilomi, M Musiani, M Zerbini, Journal of Clinical Microbiology . 1997,第6期

机译：点免疫过氧化物酶测定法可检测血清样品中的细小病毒B19抗原。
4. Rapid and Sensitive Detection of HSV in pregnant Women During Pregnancy and Before Labor By Direct Fluorescent Assay (DFA) with Monoclonal Antibodies [C] . M. Askienazy-Elbhar, M. Azoulay International Multidisciplinary Congress . 2003

机译：用单克隆抗体直接荧光测定（DFA）在妊娠期间和劳动前的孕妇HSV快速敏感
5. Characterization of Anti-Ricin Monoclonal Antibodies and the Construction of a Chimeric Murine-Human IgG2/K Anti-Ricin Monoclonal Antibody [D] . Vendramelli, Robert Matthew 2011

机译：抗蓖麻毒蛋白单克隆抗体的表征和鼠/人IgG2 / K嵌合抗蓖麻毒蛋白单克隆抗体的构建
6. Dot immunoperoxidase assay using monoclonal antibody for direct detection of cytomegalovirus in urine samples. [O] . M Zerbini, M Musiani, G Gentilomi, 1987

机译：使用单克隆抗体的点免疫过氧化物酶测定法可直接检测尿液样本中的巨细胞病毒。
7. Rapid detection of cytomegalovirus in cell culture by indirect immunoperoxidase staining with monoclonal antibody to an early nuclear antigen [O] . P D Swenson, M H Kaplan 1985

机译：间接免疫氧化酶用单克隆抗体与早期核抗原间接免疫氧化酶染色的快速检测细胞培养中的细胞培养物
8. Characterization of Monoclonal Antibodies to Two Treponema denticola Serotypes bythe Indirect Fluorescent-Antibody Assay [R] . Kearns, E. A., Simonson, L. G., Schutt, R. W., 1991

机译：通过间接荧光抗体测定表征两种密螺旋体血清型的单克隆抗体

Dot immunoperoxidase assay using monoclonal antibody for direct detection of cytomegalovirus in urine samples.

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