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首页> 外文期刊>Journal of Clinical Microbiology >Comparison of Genomic Methods for Differentiating Strains of Enterococcus faecium: Assessment Using Clinical Epidemiologic Data
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Comparison of Genomic Methods for Differentiating Strains of Enterococcus faecium: Assessment Using Clinical Epidemiologic Data

机译：区分粪肠球菌菌株的基因组方法比较：使用临床流行病学数据进行评估

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摘要

Genomic DNA extracted from 45 vancomycin-resistantEnterococcus faecium (VRE) isolates was cleaved withHindIII and HaeIII and subjected to agarose gel electrophoresis. The ability of this method (restriction endonuclease analysis [REA]) to distinguish strains at the subspecies level was compared with results previously determined by pulsed-field gel electrophoresis (PFGE). Chart reviews were performed to provide a clinical correlation of possible epidemiologic relatedness. A likely clinical association was found for 29 patients as part of two outbreaks. REA found 21 of 21 isolates were the same type in the first outbreak, with PFGE calling 19 strains the same type. In the second outbreak with eight patient isolates, HindIII found six were the same type and two were unique types. HaeIII found three strains were the same type, two strains were a separate type, and three more strains were unique types, while PFGE found three were the same type and five were unique types. No single “ideal” method can be used without clinical epidemiologic investigation, but any of these techniques is helpful in providing focus to infection control practitioners assessing possible outbreaks of nosocomial infection.
机译：用 Hin dIII和 Hae III裂解从45个耐万古霉素的粪肠球菌（VRE）分离物中提取的基因组DNA，并进行琼脂糖凝胶电泳。将这种方法（限制性核酸内切酶分析[REA]）区分亚种水平菌株的能力与先前通过脉冲场凝胶电泳（PFGE）确定的结果进行了比较。进行图表审查以提供可能的流行病学相关性的临床相关性。在两次暴发中，有29位患者可能与临床相关。 REA发现，在第一次疫情中21株分离株中有21株属于同一类型，PFGE称19株菌株为同一类型。在第二次暴发中，有8例患者分离株， Hin dIII发现6种是相同类型，而2种是独特类型。 Hae III发现三个菌株是同一类型，两个菌株是单独的类型，另外三个菌株是唯一类型，而PFGE发现三个菌株是同一类型，五个菌株是唯一类型。没有临床流行病学调查，不能使用任何单一的“理想”方法，但是这些技术中的任何一种都有助于将重点放在感染控制从业者身上，以评估医院内感染的可能爆发情况。

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