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首页> 外文期刊>Journal of Clinical Microbiology >Application of 'Hirt supernatant' DNA to the molecular epidemiology of cytomegalovirus infections.
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Application of 'Hirt supernatant' DNA to the molecular epidemiology of cytomegalovirus infections.

机译:“ Hirt上清液” DNA在巨细胞病毒感染的分子流行病学中的应用。

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摘要

Human cytomegalovirus DNA was isolated from infected cells by the Hirt method (B. Hirt, J. Mol. Biol. 26:365-369, 1967). The restriction endonuclease cleavage patterns of DNA obtained in this manner were comparable with those of DNA extracted from purified virions. The "Hirt supernatants" were satisfactory for identifying individual cytomegalovirus strains by their DNA fingerprinting.
机译:通过Hirt方法(B.Hirt,J.Mol.Biol.26:365-369,1967)从感染细胞中分离出人巨细胞病毒DNA。以这种方式获得的DNA的限制性核酸内切酶切割模式与从纯化的病毒体提取的DNA的限制性核酸内切酶切割模式相当。 “ Hirt上清液”对于通过DNA指纹图谱鉴定单个巨细胞病毒株是令人满意的。

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